{"title":"Cloning","description":null,"products":[{"product_id":"ez-c-cell-e-coli-bl21-de3-106-efficiency","title":"Ez C-cell E. coli BL21(DE3)","description":"\u003cp\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e Ez C-cell E. coli BL21(DE3) is a high-efficiency competent cell product designed for protein expression, created using a chemical modification method. Optimized for subcloning applications, this product offers a transformation efficiency of 10⁶ CFU\/µg, allowing users to quickly and accurately clone their desired genes.\u003c\/p\u003e\n\u003cp\u003eEz C-cell is provided in convenient, aliquoted units, making it easy to use. Each package includes SOC broth for recovery culture, and plating beads are also provided to facilitate easy spreading on agarplates. \u003cbr\u003e\u003cbr\u003eThis product contains an Enhancer that boosts transformation efficiency, ensuring effective and reliable results.\u003c\/p\u003e\n\u003cp\u003eEz C-cell E. coli BL21(DE3) is designed to enable users to perform cloning quickly, easily, and consistently. This simpification allows researchers to focus on their protein expression and research goals without getting bogged down in complex procedures. \u003cbr\u003e\u003cbr\u003eEz C-cell excels in various applications, including life science research, recombinant protein production, and enzyme activity studies, delivering outstanding performance. It sets a new standard in protein expression and significantly enhances research efficiency. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCharacteristics\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e• High Transformation Efficiency\u003c\/p\u003e\n\u003cp\u003e• User Convenience \u003c\/p\u003e\n\u003cp\u003e• Inclusion of Enhancer \u003c\/p\u003e\n\u003cp\u003e• Rapid Growth\u003c\/p\u003e\n\u003cp\u003e• Versatile Applications\u003c\/p\u003e\n\u003cp\u003e• Cost Efficiency\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eConvenient Product Features\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e• Competent Cells\u003c\/p\u003e\n\u003cp\u003e• SOC Broth\u003c\/p\u003e\n\u003cp\u003e• Plating Beads\u003c\/p\u003e\n\u003cp\u003e• Control Plasmid\u003cb\u003e \u003c\/b\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Contents\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; margin: 16px 0;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth style=\"border: 1px solid #d9d9d9; padding: 8px 10px; text-align: left; vertical-align: top;\"\u003eNo.\u003c\/th\u003e\n\u003cth style=\"border: 1px solid #d9d9d9; padding: 8px 10px; text-align: left; vertical-align: top;\"\u003eKit Contents\u003c\/th\u003e\n\u003cth style=\"border: 1px solid #d9d9d9; padding: 8px 10px; text-align: left; vertical-align: top;\"\u003eUnit\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px; text-align: left; vertical-align: top;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px; text-align: left; vertical-align: top;\"\u003eC-cell BL21(DE3)\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px; text-align: left; vertical-align: top;\"\u003e30 ㎕\/tube x 20 tubes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px; text-align: left; vertical-align: top;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px; text-align: left; vertical-align: top;\"\u003eEnhancer Solution\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px; text-align: left; vertical-align: top;\"\u003e500 ㎕\/tube x 1 tube\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px; text-align: left; vertical-align: top;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px; text-align: left; vertical-align: top;\"\u003eSOC broth\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px; text-align: left; vertical-align: top;\"\u003e1.7 ml\/tube\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px; text-align: left; vertical-align: top;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px; text-align: left; vertical-align: top;\"\u003ePlating bead\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px; text-align: left; vertical-align: top;\"\u003e60~70 ea\/tube x 2 tubes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px; text-align: left; vertical-align: top;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px; text-align: left; vertical-align: top;\"\u003eControl DNA\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px; text-align: left; vertical-align: top;\"\u003e50 ㎕\/tube x 1 tube\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px; text-align: left; vertical-align: top;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px; text-align: left; vertical-align: top;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px; text-align: left; vertical-align: top;\"\u003e1 ea\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e• Should be stored at or below -80°C until the expiration date indicated\u003c\/p\u003e\n\u003cp\u003e• Avoid freeze-thaw cycles and do not reuse\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAnalysis Workflow\u003c\/strong\u003e\u003cbr\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTechnical Data\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003e1. Confirmation of Protein Expression in E. coli BL21(DE3)\u003c\/b\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eRecombinant plasmids encoding DNA polymerases were transformed into Ez C-cell E. coli BL21(DE3), followed by induction of protein expression using IPTG for 4 hours.\u003c\/li\u003e\n\u003cli\u003eSDS-PAGE analysis revealed a distinct protein band at approximately 63 kDa post-IPTG induction.\u003c\/li\u003e\n\u003cli\u003eComparison of Coomassie Brilliant Blue staining before and after induction demonstrated a faint band at this position prior to induction, while a prominent band was observed around 63 kDa following induction.\u003c\/li\u003e\n\u003cli\u003eThese results confirm the potential of Ez C-cell E. coli BL21(DE3) as a suitable host for protein expression.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cb\u003e2. Effect of Spreading Beads\u003c\/b\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eEz C-cell products provide Plating beads to assist with spreading after transformation.\u003c\/li\u003e\n\u003cli\u003eFor a single use, 5 to 6 beads are added, and the plate is gently shaken to perform the spreading process.\u003c\/li\u003e\n\u003cli\u003eUsing Plating beads helps to distribute colonies more evenly across the plate, allowing for approximately 1.4 times more colonies to be observed compared to traditional methods using a spreader.\u003c\/li\u003e\n\u003cli\u003eThe product includes enough beads for 20 spreading applications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eAvailable pack options:\u003c\/strong\u003e 20T(50 μl x 20 ea). \u003cstrong\u003eManufacturer reference:\u003c\/strong\u003e 15066.\u003c\/p\u003e","brand":"iNtRON Biotechnology","offers":[{"title":"Default Title","offer_id":64724320649593,"sku":"15066","price":89.95,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0945\/3043\/7497\/files\/EzC-cellE.coliBL21_DE3.png?v=1790714912"},{"product_id":"ez-c-cell-e-coli-dh5-108-efficiency","title":"Ez C-cell E. coli DH5α","description":"\u003cp\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eEz C-cell E. coli DH5α is a high-performance Competent Cell produced using a chemical modification method, offering the optimal solution for cloning and library construction. With a transformation efficiency of 10⁸ CFU\/µg of DNA, this product is designed to enable researchers to perform DNA cloning more easily and rapidly. \u003cbr\u003e\u003cbr\u003eTo maximize user convenience, Ez C-cell is provided in individual C-cell units, allowing researchers to use only the required amount and efficiently store any leftovers. Additionally, it comes with SOC broth for recovery culture and plating beads that facilitate easy spreading on agar plates, minimizing hassle during the experimental process. These features significantly enhance experimental consistency and improve the overall user experience.\u003cbr\u003e\u003cbr\u003eOne of the standout aspects of Ez C-cell is its Superfast Protocol which reduces the traditional cloning process, typically takng 1 hour and 40 minutes, to just 5 minutes. This time-saving capability allows researchers to maximize their productivity and conduct more experiments efficently.\u003cbr\u003e\u003cbr\u003eEz C-cell E. coli DH5α supports users in achieving quick, reliable, and consistent cloning results, thereby increasing the success rate of experiments and maximizing research efficiency. This product sets a new standard for cloning workflows, promising better outcomes for researchers. \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCharacteristics\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e• High Transformation Efficiency\u003c\/p\u003e\n\u003cp\u003e• Ease of Use \u003c\/p\u003e\n\u003cp\u003e• Superfast Protocol \u003c\/p\u003e\n\u003cp\u003e• Convenient Plating Function\u003c\/p\u003e\n\u003cp\u003e• Time Savings\u003c\/p\u003e\n\u003cp\u003e• Cost Efficiency\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eConvenient Product Features\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e• Competent Cells\u003c\/p\u003e\n\u003cp\u003e• SOC Broth\u003c\/p\u003e\n\u003cp\u003e• Plating Beads\u003c\/p\u003e\n\u003cp\u003e• Control Plasmid\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Contents\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; margin: 16px 0px; height: 117.563px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003cth style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; text-align: left; vertical-align: top; height: 19.5938px;\"\u003eNo.\u003c\/th\u003e\n\u003cth style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; text-align: left; vertical-align: top; height: 19.5938px;\"\u003eKit Contents\u003c\/th\u003e\n\u003cth style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; text-align: left; vertical-align: top; height: 19.5938px;\"\u003eUnit\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; text-align: left; vertical-align: top; height: 19.5938px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; text-align: left; vertical-align: top; height: 19.5938px;\"\u003eC-cell DH5α\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; text-align: left; vertical-align: top; height: 19.5938px;\"\u003e50 ㎕\/tube\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; text-align: left; vertical-align: top; height: 19.5938px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; text-align: left; vertical-align: top; height: 19.5938px;\"\u003eSOC broth\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; text-align: left; vertical-align: top; height: 19.5938px;\"\u003e1.7 ml\/tube\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; text-align: left; vertical-align: top; height: 19.5938px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; text-align: left; vertical-align: top; height: 19.5938px;\"\u003ePlating bead\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; text-align: left; vertical-align: top; height: 19.5938px;\"\u003e60~70 ea \/ tube\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; text-align: left; vertical-align: top; height: 19.5938px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; text-align: left; vertical-align: top; height: 19.5938px;\"\u003eControl DNA\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; text-align: left; vertical-align: top; height: 19.5938px;\"\u003e50 ㎕\/tube\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; text-align: left; vertical-align: top; height: 19.5938px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; text-align: left; vertical-align: top; height: 19.5938px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; text-align: left; vertical-align: top; height: 19.5938px;\"\u003e1 ea\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e• Should be stored at or below -80°C until the expiration date indicated\u003c\/p\u003e\n\u003cp\u003e• Avoid freeze-thaw cycles and do not reuse\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAnalysis Workflow\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTechnical Data\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Transformation Efficiency of Ez C-cell E. coli DH5α \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e• An evaluation was conducted on the transformation efficiency of Ez C-cell E. coli DH5α using cloning vectors, protein expression vectors, and a long-length adenoviral-based vector.\u003c\/p\u003e\n\u003cp\u003e• The representative cloning vector, pUC19, demonstrated a transformation efficiency of approximately 1.9 x 10⁸ CFU\/µg, while the 33.4 kb long pAdEasy-1 vector showed a transformation efficiency of about 2.9 x 10⁶ CFU\/µg.\u003c\/p\u003e\n\u003cp\u003e• These results indicate performance that is equal to or exceeds that of similar products from other sources. (The above test data is provided as our experimental result, and variations may occur depending on the quality of the DNA.)\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003e2. Effect of Spreading Beads\u003c\/b\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e• Ez C-cell products provide Plating beads to assist with spreading after transformation.\u003c\/p\u003e\n\u003cp\u003e• For a single use, 5 to 6 beads are added, and the plate is gently shaken to perform the spreading process.\u003c\/p\u003e\n\u003cp\u003e• Using Plating beads helps to distribute colonies more evenly across the plate, allowing for approximately 1.4 times more colonies to be observed compared to traditional methods using a spreader.\u003c\/p\u003e\n\u003cp\u003e• The product includes enough beads for 20 spreading applications.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAvailable pack options:\u003c\/strong\u003e 20T(50 μl x 20 ea). \u003cstrong\u003eManufacturer reference:\u003c\/strong\u003e 15065.\u003c\/p\u003e","brand":"iNtRON Biotechnology","offers":[{"title":"Default Title","offer_id":64724320682361,"sku":"15065","price":89.95,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0945\/3043\/7497\/files\/EzC-cellE.coliDH5.png?v=1790714232"}],"url":"https:\/\/hayubiomedical.co.uk\/collections\/cloning.oembed","provider":"HAYU Biomedical Limited","version":"1.0","type":"link"}