{"product_id":"bf2000-smochem-1x-mops-sds-running-buffer-powder-5-packs-for-5000-ml","title":"SMOChem™ 1X MOPS-SDS Running Buffer Powder","description":"\u003cp\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSMOChem™ 1X MOPS-SDS Running Buffer Powder\u003c\/strong\u003e is a convenient, premeasured electrophoresis buffer from SMOBIO Technology for denaturing protein separation by SDS-PAGE using the Bis-Tris buffer system.\u003c\/p\u003e\n\u003cp\u003eThe buffer is designed for use with Q-PAGE™ Bis-Tris Precast Gels and other compatible polyacrylamide gels operating with MOPS-SDS running buffer. It supports reproducible protein migration and effective separation of medium- and larger-sized proteins.\u003c\/p\u003e\n\u003cp\u003eCompared with MES-SDS running buffer, MOPS-SDS generally provides improved separation of medium- to high-molecular-weight proteins. This makes it suitable for routine protein characterization, protein-purity assessment, recombinant protein analysis and sample separation before Western blotting.\u003c\/p\u003e\n\u003cp\u003eEach package contains five individually measured powder pouches. One complete pouch is dissolved in deionized water to prepare 1 litre of ready-to-use 1X MOPS-SDS buffer, providing a total preparation volume of 5 litres per product pack.\u003c\/p\u003e\n\u003cp\u003eThe prepared 1X buffer contains 50 mM Tris base, 50 mM MOPS, 0.1% SDS and 1 mM EDTA. No pH adjustment is required. The premeasured powder dissolves within minutes, reducing weighing errors and improving consistency between electrophoresis runs.\u003c\/p\u003e\n\u003cp\u003eSodium dodecyl sulfate, or SDS, denatures proteins and provides a broadly uniform negative charge-to-mass ratio. Protein migration during electrophoresis therefore depends primarily on molecular size rather than intrinsic electrical charge.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e Premeasured powder formulation • Five individually packaged pouches • Each pouch prepares 1 litre of 1X buffer • Five-litre total preparation volume • No pH adjustment required • Dissolves within minutes • Optimized for Bis-Tris SDS-PAGE • Suitable for medium- and larger-sized proteins • Standardized composition • Reliable and reproducible protein separation • Compatible with Q-PAGE™ Bis-Tris Precast Gels • Room-temperature storage\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e Bis-Tris SDS-PAGE • Denaturing Protein Electrophoresis • Q-PAGE™ Bis-Tris Gel Electrophoresis • Medium- and High-Molecular-Weight Protein Separation • Protein Molecular-Weight Analysis • Protein-Purity Assessment • Recombinant Protein Analysis • Protein Expression Studies • Western Blot Sample Separation\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFinal 1X Composition:\u003c\/strong\u003e 50 mM Tris base • 50 mM MOPS • 0.1% SDS • 1 mM EDTA\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePreparation:\u003c\/strong\u003e Empty the complete contents of one pouch into a suitable laboratory container. Add deionized water and dissolve the powder completely, then adjust the final volume to 1 litre with deionized water. No pH adjustment is required.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eImportant Preparation Note:\u003c\/strong\u003e Do not divide a powder pouch to prepare a smaller volume. Uneven distribution of the different powder components may alter the final buffer composition or pH. Always use one complete pouch to prepare 1 litre of 1X buffer.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eUse Recommendation:\u003c\/strong\u003e Use fresh buffer for consistent electrophoresis performance. Reusing running buffer may alter its pH and ionic strength and may introduce residual protein contamination that affects migration and band resolution.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e Store unopened powder pouches at room temperature. The manufacturer states a shelf life of 24 months under the recommended storage conditions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResearch Use:\u003c\/strong\u003e Research Use Only (RUO). Not intended for diagnostic or clinical use.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eManufacturer:\u003c\/strong\u003e SMOBIO Technology, Inc.\u003c\/p\u003e\n\u003ch3 style=\"margin-top: 22px; margin-bottom: 10px;\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv style=\"overflow-x: auto; width: 100%; margin-bottom: 24px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 14px; line-height: 1.4; height: 509.438px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003cth style=\"border: 1px solid rgb(217, 217, 217); background: rgb(245, 245, 245); padding: 9px 10px; text-align: left; width: 42%; height: 19.5938px;\"\u003eSpecification\u003c\/th\u003e\n\u003cth style=\"border: 1px solid rgb(217, 217, 217); background: rgb(245, 245, 245); padding: 9px 10px; text-align: left; height: 19.5938px;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e\u003cstrong\u003eCatalogue No.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003eBF2000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 39.1875px;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 39.1875px;\"\u003eSMOChem™ 1X MOPS-SDS Running Buffer Powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e\u003cstrong\u003eProduct Form\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003ePremeasured powder pouches\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e\u003cstrong\u003ePack Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e5 pouches\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e\u003cstrong\u003eVolume Prepared per Pouch\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e1 litre of 1X buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e\u003cstrong\u003eTotal Preparation Volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e5 litres\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e\u003cstrong\u003eWorking Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e1X\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e\u003cstrong\u003eBuffer System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003eMOPS-SDS for Bis-Tris gels\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e\u003cstrong\u003eTris Base Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e50 mM in the prepared 1X buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e\u003cstrong\u003eMOPS Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e50 mM in the prepared 1X buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e\u003cstrong\u003eSDS Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e0.1% in the prepared 1X buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e\u003cstrong\u003eEDTA Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e1 mM in the prepared 1X buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e\u003cstrong\u003eWater Requirement\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003eDeionized water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e\u003cstrong\u003epH Adjustment\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003eNot required\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e\u003cstrong\u003eElectrophoresis Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003eDenaturing protein electrophoresis by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e\u003cstrong\u003eCompatible Precast Gels\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003eQ-PAGE™ Bis-Tris Precast Gels\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 39.1875px;\"\u003e\u003cstrong\u003eOther Compatible Gels\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 39.1875px;\"\u003eCompatible polyacrylamide gels using the Bis-Tris buffer system\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e\u003cstrong\u003eRecommended Protein Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003eMedium- to large-sized proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 39.1875px;\"\u003e\u003cstrong\u003ePrimary Application\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 39.1875px;\"\u003eRunning buffer for SDS-PAGE in the Bis-Tris system\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e\u003cstrong\u003eStorage Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003eRoom temperature\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e\u003cstrong\u003eStated Shelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003e\u003cstrong\u003eClassification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid rgb(217, 217, 217); padding: 8px 10px; height: 19.5938px;\"\u003eResearch Use Only (RUO)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size: 13px; color: #666;\"\u003e\u003cem\u003eImportant: Use MOPS-SDS buffer with compatible Bis-Tris gels. It should not be substituted with Tris-Glycine-SDS running buffer, as using an incompatible buffer system can alter protein migration and reduce separation quality.\u003c\/em\u003e\u003c\/p\u003e","brand":"SMOBIO","offers":[{"title":"5 pouches – prepares 5 L","offer_id":64346722042233,"sku":"BF2000","price":63.99,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0945\/3043\/7497\/files\/ChatGPTImageJun15_2026_04_21_05PM.png?v=1781536901","url":"https:\/\/hayubiomedical.co.uk\/products\/bf2000-smochem-1x-mops-sds-running-buffer-powder-5-packs-for-5000-ml","provider":"HAYU Biomedical Limited","version":"1.0","type":"link"}