{"product_id":"bf4000-smochem-1x-tris-glycine-sds-running-buffer-powder-5-packs-for-5000-ml","title":"SMOChem™ 1X Tris-Glycine-SDS Running Buffer Powder","description":"\u003cp\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSMOChem™ 1X Tris-Glycine-SDS Running Buffer Powder\u003c\/strong\u003e is a convenient, premeasured electrophoresis buffer from SMOBIO Technology for separating denatured proteins by SDS-PAGE using the Tris-Glycine buffer system.\u003c\/p\u003e\n\u003cp\u003eThe buffer is designed for use with Q-PAGE™ TGN Precast Gels and conventional Laemmli Tris-HCl polyacrylamide gels. Its standardized formulation supports reliable protein migration and reproducible band separation across routine SDS-PAGE applications.\u003c\/p\u003e\n\u003cp\u003eEach package contains five premeasured pouches. One complete pouch is dissolved in deionized water to prepare 1 litre of ready-to-use 1X Tris-Glycine-SDS running buffer, providing a total preparation volume of 5 litres per product pack.\u003c\/p\u003e\n\u003cp\u003eThe prepared 1X buffer contains 25 mM Tris, 192 mM glycine and 0.10% SDS. No pH adjustment is required. The powder dissolves within minutes, reducing weighing errors and simplifying routine buffer preparation.\u003c\/p\u003e\n\u003cp\u003eSodium dodecyl sulfate, or SDS, denatures proteins and provides a broadly uniform negative charge-to-mass ratio. During electrophoresis, protein migration therefore depends primarily on molecular size rather than the proteins’ intrinsic electrical charge.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e Premeasured powder formulation • Five individually packaged pouches • Each pouch prepares 1 litre of 1X buffer • Five-litre total preparation volume • No pH adjustment required • Dissolves within minutes • Standardized composition • Reliable and reproducible protein separation • Convenient room-temperature storage • Compatible with Q-PAGE™ TGN and conventional Laemmli Tris-HCl gels\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e SDS-PAGE • Denaturing Protein Electrophoresis • Q-PAGE™ TGN Gel Electrophoresis • Laemmli Tris-HCl Gel Electrophoresis • Protein Separation • Protein Molecular-Weight Analysis • Protein-Purity Assessment • Protein Expression Analysis • Western Blot Sample Separation\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFinal 1X Composition:\u003c\/strong\u003e 25 mM Tris • 192 mM glycine • 0.10% SDS\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePreparation:\u003c\/strong\u003e Empty the entire contents of one pouch into a suitable laboratory container. Add deionized water and dissolve the powder completely, then adjust the final volume to 1 litre with deionized water. No pH adjustment is required.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eImportant Preparation Note:\u003c\/strong\u003e Do not divide a powder pouch to prepare a smaller volume. Uneven distribution of the powder components may alter the final buffer composition or pH. Always dissolve one complete pouch to prepare 1 litre of 1X running buffer.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eUse Recommendation:\u003c\/strong\u003e Use fresh running buffer for consistent electrophoresis performance. Reusing buffer may change its pH and ionic strength and may introduce residual protein contamination that affects separation quality.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e Store the unopened powder pouches at room temperature. The manufacturer states a shelf life of 24 months under the recommended storage conditions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResearch Use:\u003c\/strong\u003e Research Use Only (RUO). Not intended for diagnostic or clinical use.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eManufacturer:\u003c\/strong\u003e SMOBIO Technology, Inc.\u003c\/p\u003e\n\u003ch3 style=\"margin-top: 22px; margin-bottom: 10px;\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv style=\"overflow-x: auto; width: 100%; margin-bottom: 24px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 14px; line-height: 1.4;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"border: 1px solid #d9d9d9; background: #f5f5f5; padding: 9px 10px; text-align: left; width: 42%;\"\u003eSpecification\u003c\/th\u003e\n\u003cth style=\"border: 1px solid #d9d9d9; background: #f5f5f5; padding: 9px 10px; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eCatalogue No.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eBF4000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eSMOChem™ 1X Tris-Glycine-SDS Running Buffer Powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eProduct Form\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003ePremeasured powder pouches\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003ePack Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e5 pouches\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eVolume Prepared per Pouch\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e1 litre of 1X buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eTotal Preparation Volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e5 litres\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eWorking Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e1X\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eBuffer System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eTris-Glycine-SDS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eTris Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e25 mM in the prepared 1X buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eGlycine Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e192 mM in the prepared 1X buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eSDS Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e0.10% in the prepared 1X buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eWater Requirement\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eDeionized water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003epH Adjustment\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eNot required\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eElectrophoresis Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eDenaturing protein electrophoresis by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eCompatible Precast Gels\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eQ-PAGE™ TGN Precast Gels\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eOther Compatible Gels\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eConventional Laemmli Tris-HCl polyacrylamide gels\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003ePrimary Application\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eRunning buffer for Tris-Glycine SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eStorage Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eRoom temperature\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eStated Shelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eClassification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eResearch Use Only (RUO)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size: 13px; color: #666;\"\u003e\u003cem\u003eNote: The white fragmented or noodle-like appearance of the SDS component is normal. Ensure that all components are completely dissolved before using the prepared buffer.\u003c\/em\u003e\u003c\/p\u003e","brand":"SMOBIO","offers":[{"title":"5 pouches – prepares 5 L","offer_id":64346697630073,"sku":"BF4000","price":63.99,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0945\/3043\/7497\/files\/ChatGPTImageJun15_2026_02_09_16PM.png?v=1781528973","url":"https:\/\/hayubiomedical.co.uk\/products\/bf4000-smochem-1x-tris-glycine-sds-running-buffer-powder-5-packs-for-5000-ml","provider":"HAYU Biomedical Limited","version":"1.0","type":"link"}