{"product_id":"exceltaq-5x-fluorescent-pcr-master-mix-tp1260","title":"ExcelTaq™ 5X Fluorescent PCR Master Mix, 200 RXN","description":"\u003cp\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eExcelTaq™ 5X Fluorescent PCR Master Mix\u003c\/strong\u003e is a ready-to-use PCR mixture containing the essential amplification components, bromophenol blue tracking dye and a fluorescent DNA stain. Only primers, template DNA and water need to be added.\u003c\/p\u003e\n\n\u003cp\u003eAmplified products can be loaded directly onto TAE- or TBE-buffered agarose gels and visualised using 470 nm blue light or UV illumination without a separate DNA-staining step.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e 5X ready-to-use formulation • Built-in fluorescent DNA stain • Contains bromophenol blue tracking dye • Direct agarose-gel loading • Immediate DNA visualisation • Compatible with blue-light and UV illumination • High amplification yield and reproducibility • Reduced pipetting steps • Produces 3′-dA overhangs\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e Routine PCR • Colony PCR • High-Throughput PCR • DNA Amplification up to 8 kb • Direct Gel Electrophoresis • TA Cloning • DNA Labelling • Molecular Biology Research\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Suitable for agarose-gel electrophoresis using TAE or TBE running buffer. PCR products may be visualised using a 470 nm blue-light illuminator or conventional UV transilluminator.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003ePackaging:\u003c\/strong\u003e Supplied as two 1 mL tubes, providing 2 mL total and sufficient reagent for approximately 200 reactions.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eManufacturer:\u003c\/strong\u003e SMOBIO Technology\u003c\/p\u003e\n\n\u003ch3 style=\"margin-top:22px; margin-bottom:10px;\"\u003eTechnical Specifications\u003c\/h3\u003e\n\n\u003cdiv style=\"overflow-x:auto; width:100%; margin-bottom:24px;\"\u003e\n\u003ctable style=\"width:100%; border-collapse:collapse; font-size:14px; line-height:1.4;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"border:1px solid #d9d9d9; background:#f5f5f5; padding:9px 10px; text-align:left; width:42%;\"\u003eSpecification\u003c\/th\u003e\n\u003cth style=\"border:1px solid #d9d9d9; background:#f5f5f5; padding:9px 10px; text-align:left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eTP1260\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eExcelTaq™ 5X Fluorescent PCR Master Mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eReady-to-use fluorescent PCR master mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e5X\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003ePack Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e1 mL × 2 tubes; 2 mL total\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eNumber of Reactions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eApproximately 200 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eTracking Dye\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eBromophenol blue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eDNA-Staining Dye\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eIntegrated fluorescent DNA stain\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eSeparate Gel Staining Required\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eNo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eDirect Gel Loading\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eYes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eCompatible Running Buffers\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eTAE and TBE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eVisualisation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e470 nm blue light or UV illumination\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003e5′→3′ Polymerase Activity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eYes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003e3′→5′ Exonuclease Activity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eNot detectable; no proofreading activity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003ePCR Product Ends\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e3′-dA overhangs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eRecommended Maximum Target Length\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eUp to 8 kb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eTypical Reaction Volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e50 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eMaster Mix per 50 µL Reaction\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e10 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eRecommended Template Amount\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e1–150 ng per 50 µL reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eRecommended Primer Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e0.1–0.5 µM each\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eTypical Initial Denaturation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e94°C for 2 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eTypical Cycling\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e25–40 cycles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eTypical Denaturation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e94°C for 30 seconds\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eTypical Annealing\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e50–68°C for 30 seconds\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eTypical Extension\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e72°C for approximately 30 seconds per kb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eStorage at 4°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eUp to 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eStorage at −20°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eUp to 24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eStorage Precautions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eProtect from light and minimise repeated freeze–thaw cycles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eManufacturer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eSMOBIO Technology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eClassification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eResearch Use Only (RUO)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\n\u003cp style=\"font-size:13px; color:#666;\"\u003e\u003cem\u003eNote: At low DNA concentrations, the fluorescent dye-to-DNA ratio may reduce migration speed and cause products to appear larger than expected. This master mix is not recommended when precise discrimination between similarly sized DNA fragments is required.\u003c\/em\u003e\u003c\/p\u003e","brand":"SMOBIO","offers":[{"title":"Default Title","offer_id":56871487275385,"sku":"TP1260","price":99.99,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0945\/3043\/7497\/files\/ChatGPTImageJun15_2026_06_18_13PM.png?v=1781543908","url":"https:\/\/hayubiomedical.co.uk\/products\/exceltaq-5x-fluorescent-pcr-master-mix-tp1260","provider":"HAYU Biomedical Limited","version":"1.0","type":"link"}