{"product_id":"exceltaq-hot-start-ii-dna-polymerase-tp5000","title":"ExcelTaq™ Hot Start II DNA Polymerase, 500 Units","description":"\u003cp\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eExcelTaq™ Hot Start II DNA Polymerase\u003c\/strong\u003e combines recombinant thermostable Taq DNA polymerase with an aptamer-based inhibitor to minimise nonspecific amplification and primer-dimer formation during PCR setup.\u003c\/p\u003e\n\n\u003cp\u003eThe reversible inhibitor suppresses polymerase activity below 45°C and releases the enzyme during normal thermal cycling. This permits room-temperature setup without requiring an additional prolonged activation step.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e Aptamer-based Hot-Start PCR • Reversible enzyme inhibition • No prolonged activation step • Room-temperature reaction setup • High specificity and yield • Reduced nonspecific amplification • Reduced primer-dimer formation • Amplification up to 10 kb • Sensitivity down to 1 fg of plasmid DNA • Supplied with 10X HS Buffer\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e High-Specificity PCR • Routine PCR • Multiplex PCR • Colony PCR • RT-PCR Amplification • Low-Copy Template Detection • Long-Amplicon PCR • TA Cloning • Molecular Biology Research\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Suitable for standard thermal cyclers and PCR workflows requiring increased specificity or sensitivity. Primers, template DNA and dNTPs must be supplied separately.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003ePackaging:\u003c\/strong\u003e ExcelTaq™ Hot Start II DNA Polymerase, 5 U\/µL, 100 µL • 10X HS Buffer, 1 mL × 2 tubes • 500 total enzyme units.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eManufacturer:\u003c\/strong\u003e SMOBIO Technology\u003c\/p\u003e\n\n\u003ch3 style=\"margin-top:22px; margin-bottom:10px;\"\u003eTechnical Specifications\u003c\/h3\u003e\n\n\u003cdiv style=\"overflow-x:auto; width:100%; margin-bottom:24px;\"\u003e\n\u003ctable style=\"width:100%; border-collapse:collapse; font-size:14px; line-height:1.4;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"border:1px solid #d9d9d9; background:#f5f5f5; padding:9px 10px; text-align:left; width:42%;\"\u003eSpecification\u003c\/th\u003e\n\u003cth style=\"border:1px solid #d9d9d9; background:#f5f5f5; padding:9px 10px; text-align:left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eTP5000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eExcelTaq™ Hot Start II DNA Polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eEnzyme Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eRecombinant thermostable Hot-Start Taq DNA polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eHot-Start Mechanism\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eReversible aptamer-based inhibition\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eInhibition Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003ePolymerase is inhibited below 45°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eEnzyme Activation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eReleased during normal PCR cycling; no prolonged activation step required\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eEnzyme Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e5 U\/µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eEnzyme Volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e100 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eTotal Enzyme Quantity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e500 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eMaximum Amplicon Length\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eUp to 10 kb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eDemonstrated Plasmid Sensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eAs low as 1 fg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eDemonstrated cDNA Sensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eAs low as 1 pg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eRoom-Temperature Setup\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eSuitable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003e10X HS Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e1 mL × 2 tubes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003e10X HS Buffer Composition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e200 mM Tris-HCl, 100 mM KCl, 100 mM (NH₄)₂SO₄, 20 mM MgCl₂ and 1% Triton X-100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003edNTPs Included\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eNo; add separately\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eTypical Reaction Volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e50 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eRecommended Template Amount\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e1–150 ng; optimise according to template source and quality\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eRecommended Primer Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e0.1–0.5 µM each\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eRecommended dNTP Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e0.2 mM each\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003e10X HS Buffer per Reaction\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e5 µL per 50 µL reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eRecommended Enzyme Amount\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e1.25 U; 0.25 µL per 50 µL reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eInitial Denaturation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e94°C for 2 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eTypical Cycling\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e25–40 cycles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eDenaturation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e94°C for 30 seconds\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eAnnealing\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e50–68°C for 30 seconds; optimise for the selected primers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eExtension\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e72°C for approximately 30 seconds per kb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eFinal Extension\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e72°C for 1 minute\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eOne unit incorporates 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eStorage Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e−20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eStated Shelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e24 months at −20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eManufacturer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eSMOBIO Technology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eClassification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eResearch Use Only (RUO)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\n\u003cp style=\"font-size:13px; color:#666;\"\u003e\u003cem\u003eNote: The stated 10 kb amplification range and low-template sensitivity are assay-dependent. Template quality, primer design, target composition and cycling conditions may affect performance.\u003c\/em\u003e\u003c\/p\u003e","brand":"SMOBIO","offers":[{"title":"Default Title","offer_id":56871510212985,"sku":"TP5000","price":149.99,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0945\/3043\/7497\/files\/ChatGPTImageJun15_2026_06_01_47PM.png?v=1781542924","url":"https:\/\/hayubiomedical.co.uk\/products\/exceltaq-hot-start-ii-dna-polymerase-tp5000","provider":"HAYU Biomedical Limited","version":"1.0","type":"link"}