{"product_id":"g-hifi-dna-polymerase-tf3000","title":"G-HiFi™ DNA Polymerase, 1 U\/µL, 100 U","description":"\u003cp\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eG-HiFi™ DNA Polymerase\u003c\/strong\u003e is a genetically modified recombinant polymerase with an elongation enhancer for high-fidelity amplification of long and GC-rich DNA templates.\u003c\/p\u003e\n\n\u003cp\u003eThe enzyme provides approximately 70-fold higher fidelity than Taq, 3′→5′ proofreading activity and amplification of suitable targets up to 40 kb. It generates blunt-ended PCR products.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e Approximately 70-fold higher fidelity than Taq • 3′→5′ proofreading activity • Suitable for GC-rich templates • Amplification up to 40 kb • Approximately 7-second-per-kb synthesis rate • Blunt-ended products • High thermostability • Optimised 5X buffer • Reduced nonspecific amplification\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e High-Fidelity PCR • Long-Range PCR • GC-Rich Template Amplification • Difficult-Template PCR • Gene Cloning • Blunt-End Cloning • DNA Sequencing Template Preparation • Site-Directed Mutagenesis • Genomic DNA Amplification\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Suitable for PCR applications requiring accurate amplification of long, GC-rich or otherwise challenging DNA templates. Cycling conditions should be optimised for the target length, GC content and primer system.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003ePackaging:\u003c\/strong\u003e 100 µL enzyme at 1 U\/µL, providing 100 units. Supplied with 5X G-HiFi™ Buffer and dNTP Mix.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eManufacturer:\u003c\/strong\u003e SMOBIO Technology\u003c\/p\u003e\n\n\u003ch3 style=\"margin-top:22px; margin-bottom:10px;\"\u003eTechnical Specifications\u003c\/h3\u003e\n\n\u003cdiv style=\"overflow-x:auto; width:100%; margin-bottom:24px;\"\u003e\n\u003ctable style=\"width:100%; border-collapse:collapse; font-size:14px; line-height:1.4;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"border:1px solid #d9d9d9; background:#f5f5f5; padding:9px 10px; text-align:left; width:42%;\"\u003eSpecification\u003c\/th\u003e\n\u003cth style=\"border:1px solid #d9d9d9; background:#f5f5f5; padding:9px 10px; text-align:left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eTF3000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eG-HiFi™ DNA Polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eHigh-fidelity, long-range DNA polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eEnzyme Source\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eGenetically modified recombinant DNA polymerase with elongation enhancer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eEnzyme Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e1 U\/µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eTotal Enzyme Quantity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e100 U\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eEnzyme Volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e100 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003e5′→3′ DNA Polymerase Activity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eYes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003e3′→5′ Exonuclease Activity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eYes; proofreading activity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eRelative Fidelity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eApproximately 70 times higher than Taq DNA polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003ePCR Product Ends\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eBlunt ends\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eMaximum Demonstrated Target\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eUp to 40 kb from λDNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eGC-Rich Template Capability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eSuitable; performance demonstrated with a 71% GC template\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eMaximum DNA Synthesis Rate\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eApproximately 1 kb per 7 seconds\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eRecommended Extension Time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e10–30 seconds per kb at 68°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eThermostability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eGreater than 10 hours at 95°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eTypical Reaction Volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e50 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eRecommended Enzyme per Reaction\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e0.5–1 U\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eRecommended Enzyme for Targets ≤2 kb\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e0.5 U per 50 µL reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eRecommended Template Amount\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e1–150 ng per 50 µL reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eRecommended Primer Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e0.1–0.5 µM each\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003ePrimer Concentration for Targets ≥10 kb\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e0.1 µM each\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eDenaturation Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e98°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eExtension Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e68°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003e5X G-HiFi™ Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e1,200 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003edNTP Mix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e2 mM each, 600 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eApproximate Enzyme Reaction Capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e100 reactions at 1 U or up to 200 reactions at 0.5 U\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eSupporting Reagent Capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eApproximately 120 standard 50 µL reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eEnzyme Storage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e50 mM Tris-HCl, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabiliser and 50% glycerol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eAmount incorporating 10 nmol dNTP into acid-insoluble material in 30 minutes at 74°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eStorage Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e−20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eStated Stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e24 months at −20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eManufacturer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eSMOBIO Technology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eClassification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eResearch Use Only (RUO)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\n\u003cp style=\"font-size:13px; color:#666;\"\u003e\u003cem\u003eNote: The 40 kb amplification result was demonstrated using λDNA and is template-dependent. Long or GC-rich targets require careful primer design, high-quality template DNA and optimised cycling conditions.\u003c\/em\u003e\u003c\/p\u003e","brand":"SMOBIO","offers":[{"title":"Default Title","offer_id":56871448052089,"sku":"TF3000","price":99.99,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0945\/3043\/7497\/files\/ChatGPTImageJun15_2026_10_20_12PM.png?v=1781558429","url":"https:\/\/hayubiomedical.co.uk\/products\/g-hifi-dna-polymerase-tf3000","provider":"HAYU Biomedical Limited","version":"1.0","type":"link"}