{"product_id":"gamborg-b5-medium-g0209","title":"Gamborg B5 medium","description":"\u003cp\u003e\u003cstrong\u003eGamborg B5 medium\u003c\/strong\u003e by Duchefa Biochemie is supplied for plant tissue culture and laboratory research.\u003c\/p\u003e\u003ch3\u003eDescription and application\u003c\/h3\u003e\u003cp\u003eB5 medium has been defined for the growth of cell suspensions of soybean root cells in the presence of 2,4 D. Nitrate was required in a concentration of 20-30 mM. An addition of 2 mM ammoniumsulphate led to an increase in cellgrowth. NH4+ when added as the sole source of nitrogen, did not support growth. Similar results were obtai-ned when NH4NO3 was substi-tuted for (NH4)2SO4. However, ammonium ions depressed growth when the concen-tration excee-ded 2 mM. Variati-ons in the concentrations of phosphate, calcium and magnesi-um resulted in relatively minor chan-ges in growth rate. Thiamine is known to be an essenti-al nutrient for cell growth and is increa-sed in concentrati-on up to 10 mg\/l. B5 medium has been defined for the growth of cell suspensions of soybean root cells in the presence of 2,4 D. Nitrate was required in a concentration of 20-30 mM. An addition of 2 mM ammoniumsulphate led to an increase in cellgrowth. NH4+ when added as the sole source of nitrogen, did not support growth. Similar results were obtai-ned when NH4NO3 was substi-tuted for (NH4)2SO4. However, ammonium ions depressed growth when the concen-tration excee-ded 2 mM. Variati-ons in the concentrations of phosphate, calcium and magnesi-um resulted in relatively minor chan-ges in growth rate. Thiamine is known to be an essenti-al nutrient for cell growth and is increa-sed in concentrati-on up to 10 mg\/l. B5 medium has been defined for the growth of cell suspensions of soybean root cells in the presence of 2,4 D. Nitrate was required in a concentration of 20-30 mM. An addition of 2 mM ammoniumsulphate led to an increase in cellgrowth. NH4+ when added as the sole source of nitrogen, did not support growth. Similar results were obtai-ned when NH4NO3 was substi-tuted for (NH4)2SO4. However, ammonium ions depressed growth when the concen-tration excee-ded 2 mM. Variati-ons in the concentrations of phosphate, calcium and magnesi-um resulted in relatively minor chan-ges in growth rate. Thiamine is known to be an essenti-al nutrient for cell growth and is increa-sed in concentrati-on up to 10 mg\/l.\u003c\/p\u003e\u003ch3\u003eComposition and technical specifications\u003c\/h3\u003e\u003ctable class=\"table table-hover\"\u003e\n\t\t\t\t\t\t\u003cthead\u003e\n\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\u003cth class=\"col-sm-6\"\u003eMicro Elements\u003c\/th\u003e\n\t\t\t\t\t\t\t\t\u003cth class=\"col-sm-3\"\u003emg\/l\u003c\/th\u003e\n\t\t\t\t\t\t\t\t\u003cth class=\"col-sm-3\"\u003eµM\u003c\/th\u003e\n\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\u003c\/thead\u003e\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\u003ctbody\u003e\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eCoCl\u003csub\u003e2\u003c\/sub\u003e.6H\u003csub\u003e2\u003c\/sub\u003eO\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e0.025\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e0.11\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eCuSO\u003csub\u003e4\u003c\/sub\u003e.5H\u003csub\u003e2\u003c\/sub\u003eO\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e0.025\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e0.10\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eFeNaEDTA\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e36.70\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e100.00\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eH\u003csub\u003e3\u003c\/sub\u003eBO\u003csub\u003e3\u003c\/sub\u003e\n\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e3.00\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e48.52\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eKI\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e0.75\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e4.52\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eMnSO\u003csub\u003e4\u003c\/sub\u003e.H\u003csub\u003e2\u003c\/sub\u003eO\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e10.00\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e59.16\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eNa\u003csub\u003e2\u003c\/sub\u003eMoO\u003csub\u003e4\u003c\/sub\u003e.2H\u003csub\u003e2\u003c\/sub\u003eO\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e0.25\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e1.03\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eZnSO\u003csub\u003e4\u003c\/sub\u003e.7H\u003csub\u003e2\u003c\/sub\u003eO\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e2.00\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e6.96\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\u003c\/tbody\u003e\n\t\t\t\t\t\u003c\/table\u003e\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\u003ctable class=\"table table-hover\"\u003e\n\t\t\t\t\t\t\u003cthead\u003e\n\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\u003cth class=\"col-sm-6\"\u003eMacro Elements\u003c\/th\u003e\n\t\t\t\t\t\t\t\t\u003cth class=\"col-sm-3\"\u003emg\/l\u003c\/th\u003e\n\t\t\t\t\t\t\t\t\u003cth class=\"col-sm-3\"\u003emM\u003c\/th\u003e\n\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\u003c\/thead\u003e\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\u003ctbody\u003e\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eCaCl\u003csub\u003e2\u003c\/sub\u003e\n\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e113.23\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e1.02\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eKNO\u003csub\u003e3\u003c\/sub\u003e\n\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e2500.00\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e24.73\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eMgSO\u003csub\u003e4\u003c\/sub\u003e\n\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e121.56\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e1.01\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eNaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e\n\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e130.44\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e1.09\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003e(NH\u003csub\u003e4\u003c\/sub\u003e)2SO\u003csub\u003e4\u003c\/sub\u003e\n\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e134.00\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e1.01\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\u003c\/tbody\u003e\n\t\t\t\t\t\u003c\/table\u003e\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\u003cp\u003eGamborg O.L., Miller R.A., Ojima K., Nutrient requirement of suspensions cultures of soybean root cells. Exp. Cell Res., 50, 151 (1968).\u003c\/p\u003e\n\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ch4\u003eHazard statements\u003c\/h4\u003e\n\u003cul class=\"details\"\u003e\n\t\u003cli\u003e\n\u003cspan class=\"label label-warning\"\u003eH315\u003c\/span\u003e  Causes skin irritation\n\u003c\/li\u003e\n\t\u003cli\u003e\n\u003cspan class=\"label label-warning\"\u003eH319\u003c\/span\u003e  Causes serious eye irritation\n\u003c\/li\u003e\n\t\u003cli\u003e\n\u003cspan class=\"label label-warning\"\u003eH335\u003c\/span\u003e  May cause respiratory irritation\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ch4\u003ePrecautionary statements\u003c\/h4\u003e\n\u003cul class=\"details\"\u003e\n\t\u003cli\u003e\n\u003cspan class=\"label label-warning\"\u003eP261\u003c\/span\u003e  Avoid breathing dust\/fume\/gas\/mist\/vapours\/spray\n\u003c\/li\u003e\n\t\u003cli\u003e\n\u003cspan class=\"label label-warning\"\u003eP302\u003c\/span\u003e  IF ON SKIN:\n\u003c\/li\u003e\n\t\u003cli\u003e\n\u003cspan class=\"label label-warning\"\u003eP305\u003c\/span\u003e  IF IN EYES:\n\u003c\/li\u003e\n\t\u003cli\u003e\n\u003cspan class=\"label label-warning\"\u003eP351\u003c\/span\u003e  Rinse continuously with water for several minutes\n\u003c\/li\u003e\n\t\u003cli\u003e\n\u003cspan class=\"label label-warning\"\u003eP352\u003c\/span\u003e  Wash with soap and water\n\u003c\/li\u003e\n\u003c\/ul\u003e\t\n\t\t\t\t\t\t\t\u003cp\u003e\u003cstrong\u003eAvailable pack options:\u003c\/strong\u003e 5 l, 10 l, 25 l, 50 l. \u003cstrong\u003eManufacturer reference:\u003c\/strong\u003e G0209.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/duchefa-biochemie.com\/msds\/download\/number\/G0209\/name\/gamborg-b5-medium\/id\/1949\"\u003eSafety data sheet\u003c\/a\u003e\u003c\/p\u003e","brand":"Duchefa Biochemie","offers":[{"title":"5 l","offer_id":64645425725817,"sku":"G0209.0005","price":11.95,"currency_code":"GBP","in_stock":true},{"title":"10 l","offer_id":64645425758585,"sku":"G0209.0010","price":18.95,"currency_code":"GBP","in_stock":true},{"title":"25 l","offer_id":64645425791353,"sku":"G0209.0025","price":34.95,"currency_code":"GBP","in_stock":true},{"title":"50 l","offer_id":64645425824121,"sku":"G0209.0050","price":57.95,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0945\/3043\/7497\/files\/G0209_f028e717-32fc-43c1-a588-1b6a25094996.png?v=1789839713","url":"https:\/\/hayubiomedical.co.uk\/products\/gamborg-b5-medium-g0209","provider":"HAYU Biomedical Limited","version":"1.0","type":"link"}