{"product_id":"i-pfu™-dna-polymerase-with-buffer-dntps","title":"i-Pfu™ DNA Polymerase with Buffer, dNTPs","description":"\u003cp\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ei-Pfu™ DNA Polymerase with Buffer, dNTPs\u003c\/strong\u003e is a high-fidelity, thermostable DNA polymerase from iNtRON Biotechnology designed for PCR applications requiring accurate DNA synthesis. The enzyme is derived from \u003cem\u003ePyrococcus furiosus\u003c\/em\u003e DNA polymerase and catalyses DNA synthesis in the 5′→3′ direction.\u003c\/p\u003e\n\u003cp\u003ei-Pfu™ DNA Polymerase possesses 3′→5′ exonuclease proofreading activity, allowing incorrectly incorporated nucleotides to be removed during DNA synthesis. This produces PCR products with greater sequence accuracy than conventional Taq DNA polymerase and makes the enzyme particularly suitable for cloning, expression studies, mutagenesis and amplification of templates intended for DNA sequencing.\u003c\/p\u003e\n\u003cp\u003eThe polymerase produces blunt-ended amplification products and is recommended for high-fidelity amplification of genomic DNA and cDNA targets up to 10 kb. It can be used with a variety of DNA templates, including cloned fragments, plasmid DNA, phage DNA, cDNA and mammalian genomic DNA.\u003c\/p\u003e\n\u003cp\u003eThe product is supplied at a concentration of 2.5 U\/µL together with 10X PCR Buffer containing Mg²⁺, 10X Mg²⁺-free PCR Buffer, dNTP mixture and a separate MgCl₂ solution. These components provide flexibility for optimization of magnesium concentration and other PCR conditions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e High-fidelity DNA amplification • 3′→5′ exonuclease proofreading activity • Low nucleotide-incorporation error rate • Blunt-ended PCR products • Amplification of targets up to 10 kb • Suitable for genomic DNA and cDNA • Flexible magnesium optimization • Supplied with PCR buffers, dNTPs and MgCl₂ • Suitable for applications requiring accurate DNA synthesis\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e High-Fidelity PCR • Genomic DNA Amplification • cDNA Amplification • Gene Cloning • Blunt-End Cloning • DNA Sequencing Template Preparation • Gene Expression Studies • Site-Directed Mutagenesis • Primer Extension • General Molecular Biology Research\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIncluded Components:\u003c\/strong\u003e i-Pfu™ DNA Polymerase (2.5 U\/µL) • 10X PCR Buffer with 15 mM Mg²⁺ • 10X Mg²⁺-free PCR Buffer • 10 mM dNTP Mixture (2.5 mM each) • 25 mM MgCl₂ Solution\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e Store at −20°C. Under the recommended storage conditions, the manufacturer states that the product is stable for at least one year.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResearch Use:\u003c\/strong\u003e Research Use Only (RUO). Not intended for diagnostic or clinical use.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eManufacturer:\u003c\/strong\u003e iNtRON Biotechnology\u003c\/p\u003e\n\u003ch3 style=\"margin-top: 22px; margin-bottom: 10px;\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv style=\"overflow-x: auto; width: 100%; margin-bottom: 24px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 14px; line-height: 1.4;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"border: 1px solid #d9d9d9; background: #f5f5f5; padding: 9px 10px; text-align: left; width: 42%;\"\u003eSpecification\u003c\/th\u003e\n\u003cth style=\"border: 1px solid #d9d9d9; background: #f5f5f5; padding: 9px 10px; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eCatalogue No.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e25181\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003ePack Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e250 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eEnzyme\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003ei-Pfu™ DNA Polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eEnzyme Source\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eRecombinant enzyme based on \u003cem\u003ePyrococcus furiosus\u003c\/em\u003e DNA polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eEnzyme Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e2.5 U\/µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003ePolymerase Activity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e5′→3′ DNA synthesis\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003e3′→5′ Exonuclease Activity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eYes; proofreading activity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003e5′→3′ Exonuclease Activity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eNot detectable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003ePCR Product Ends\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eBlunt ends\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eRecommended Target Length\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eUp to 10 kb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eRecommended Reaction Volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e50 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eRecommended Enzyme per Reaction\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e1.25–2.5 units for a typical 50 µL reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003e10X PCR Buffer with Mg²⁺\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e1 mL; contains 15 mM Mg²⁺ at 10X concentration\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003e10X Mg²⁺-Free PCR Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003edNTP Mixture\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e10 mM formulation; 2.5 mM of each dNTP, 500 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eMgCl₂ Solution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e25 mM, 1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eRecommended Extension Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e65–72°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eStorage Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e−20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eClassification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eResearch Use Only (RUO)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e","brand":"iNtRON Biotechnology","offers":[{"title":"250 Units","offer_id":64326839140729,"sku":"25181","price":145.45,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0945\/3043\/7497\/files\/ChatGPTImageAug21_2026_04_47_38PM.png?v=1787327294","url":"https:\/\/hayubiomedical.co.uk\/products\/i-pfu%e2%84%a2-dna-polymerase-with-buffer-dntps","provider":"HAYU Biomedical Limited","version":"1.0","type":"link"}