{"product_id":"i-taq™-dna-polymerase-with-buffer-and-dntps","title":"i-Taq™ DNA Polymerase with Buffer and dNTPs","description":"\u003cp\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ei-Taq™ DNA Polymerase with Buffer and dNTPs\u003c\/strong\u003e from iNtRON Biotechnology is a highly purified, thermostable DNA polymerase for routine PCR amplification of DNA targets up to approximately 5 kb.\u003c\/p\u003e\n\u003cp\u003ei-Taq™ is a recombinant 94 kDa Taq DNA polymerase derived from the polymerase gene of \u003cem\u003eThermus aquaticus\u003c\/em\u003e strain YT-1 and expressed in \u003cem\u003eE. coli\u003c\/em\u003e. The purification process is designed to remove residual host-derived proteins and DNA that could otherwise contribute to contamination or non-specific amplification.\u003c\/p\u003e\n\u003cp\u003eThe enzyme is supplied at a concentration of 5 U\/µL together with a 10X PCR Buffer containing MgCl₂, a separate 10X MgCl₂-free PCR Buffer, a dNTP mixture and 25 mM MgCl₂. This flexible component format allows users to perform standard PCR using the MgCl₂-containing buffer or optimise the magnesium concentration for demanding templates and primer sets using the MgCl₂-free buffer and separately supplied MgCl₂ solution.\u003c\/p\u003e\n\u003cp\u003ei-Taq™ DNA Polymerase has optimal DNA synthesis activity at approximately 72°C. It is suitable for amplification from genomic DNA and cDNA templates, routine PCR, RT-PCR-derived cDNA amplification, direct sequencing-related PCR, T\/A cloning and PCR assays used for loss-of-heterozygosity or microsatellite-instability research.\u003c\/p\u003e\n\u003cp\u003eAs a conventional Taq DNA polymerase, i-Taq™ does not possess 3′→5′ proofreading exonuclease activity and typically produces PCR products with 3′-adenine overhangs. This makes the amplified products suitable for T\/A cloning. For applications requiring substantially higher sequence fidelity, a proofreading polymerase such as i-pfu™ or an enzyme blend such as i-MAX™ II should be considered.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e Standard recombinant Taq DNA polymerase • 94 kDa thermostable enzyme • 5 U\/µL concentration • Amplifies DNA targets up to approximately 5 kb • Optimal extension at approximately 72°C • Supplied with dNTP mixture • Includes MgCl₂-containing and MgCl₂-free PCR buffers • Separate MgCl₂ solution for optimisation • Suitable for genomic DNA and cDNA templates • Produces PCR products suitable for T\/A cloning • Flexible individual-component format\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTemplate Types:\u003c\/strong\u003e Genomic DNA • cDNA • Plasmid DNA • Purified DNA • Cloned DNA Templates • Other Compatible DNA Templates\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e Routine PCR • Genomic DNA PCR • cDNA Amplification • RT-PCR-Related Amplification • Colony PCR • Direct Sequencing-Related PCR • T\/A Cloning • Loss-of-Heterozygosity Research • Microsatellite-Instability Research • Other Standard PCR Applications\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResearch Use:\u003c\/strong\u003e Research Use Only (RUO)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eManufacturer:\u003c\/strong\u003e iNtRON Biotechnology\u003c\/p\u003e\n\u003ch3 style=\"margin-top: 22px; margin-bottom: 10px;\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv style=\"overflow-x: auto; width: 100%; margin-bottom: 24px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 14px; line-height: 1.4;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"border: 1px solid #d9d9d9; background: #f5f5f5; padding: 9px 10px; text-align: left; width: 42%;\"\u003eSpecification\u003c\/th\u003e\n\u003cth style=\"border: 1px solid #d9d9d9; background: #f5f5f5; padding: 9px 10px; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003ei-Taq™ DNA Polymerase with Buffer and dNTPs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eCatalogue Nos.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e25021 \/ 25022\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eAvailable Pack Sizes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e250 units \/ 500 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eEnzyme Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e5 U\/µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eEnzyme Molecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eApproximately 94 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eEnzyme Source\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eRecombinant \u003cem\u003eThermus aquaticus\u003c\/em\u003e YT-1 DNA polymerase expressed in \u003cem\u003eE. coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eMaximum Amplicon Length\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eUp to approximately 5 kb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eOptimal Extension Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eApproximately 72°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eRecommended Extension Time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eApproximately 1 minute per kb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eProofreading Activity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eNo 3′→5′ proofreading exonuclease activity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003ePCR Product Ends\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eTypically generates 3′-adenine overhangs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eCloning Compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eSuitable for T\/A cloning\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eSupplied PCR Buffers\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e10X PCR Buffer with 20 mM MgCl₂ and 10X MgCl₂-free PCR Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003edNTP Mixture\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e10 mM total mixture, 2.5 mM of each dNTP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eSeparate MgCl₂ Solution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e25 mM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eHot-Start Capability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eNo; standard Taq DNA polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e–20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eClassification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eResearch Use Only (RUO)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eManufacturer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eiNtRON Biotechnology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top: 22px; margin-bottom: 10px;\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv style=\"overflow-x: auto; width: 100%; margin-bottom: 24px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 14px; line-height: 1.4;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"border: 1px solid #d9d9d9; background: #f5f5f5; padding: 9px 10px; text-align: left;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"border: 1px solid #d9d9d9; background: #f5f5f5; padding: 9px 10px; text-align: left;\"\u003e250 Units – 25021\u003c\/th\u003e\n\u003cth style=\"border: 1px solid #d9d9d9; background: #f5f5f5; padding: 9px 10px; text-align: left;\"\u003e500 Units – 25022\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003ei-Taq™ DNA Polymerase, 5 U\/µL\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e250 units\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e500 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003e10X PCR Buffer with 20 mM MgCl₂\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e1 mL\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003e10X MgCl₂-Free PCR Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e1 mL\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003edNTP Mixture, 2.5 mM Each\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e500 µL\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003e25 mM MgCl₂\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e1 mL\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size: 13px; color: #666;\"\u003e\u003cem\u003eNote: Maximum amplicon length and PCR performance depend on template quality, target GC content, primer design, magnesium concentration, cycle conditions and reaction optimisation. For high-fidelity cloning or sequence-critical applications, use an appropriate proofreading polymerase.\u003c\/em\u003e\u003c\/p\u003e","brand":"iNtRON Biotechnology","offers":[{"title":"250 Units","offer_id":64326711935353,"sku":"25021","price":58.45,"currency_code":"GBP","in_stock":true},{"title":"500 Units","offer_id":64326711968121,"sku":"25022","price":99.95,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0945\/3043\/7497\/files\/ChatGPTImageAug21_2026_04_29_03PM.png?v=1787326158","url":"https:\/\/hayubiomedical.co.uk\/products\/i-taq%e2%84%a2-dna-polymerase-with-buffer-and-dntps","provider":"HAYU Biomedical Limited","version":"1.0","type":"link"}