{"product_id":"jena-pp-204","title":"Plasmid Mini-Prep Kit","description":"\u003cdiv style=\"border-left:3px solid #097e7d;background:#f2f8f8;padding:10px 14px;margin:0 0 18px;font-size:.92em\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO).\u003c\/strong\u003e Not for diagnostic or therapeutic use.\u003c\/div\u003e\n\u003cp style=\"font-size:1.06em;line-height:1.7\"\u003eSpin-column purification of plasmid DNA for laboratory workflows.\u003c\/p\u003e\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\u003cdiv style=\"overflow-x:auto\"\u003e\u003ctable style=\"width:100%;border-collapse:collapse;margin:0 0 8px\"\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"text-align:left;vertical-align:top;padding:12px 16px 12px 0;width:32%;font-weight:600;border-bottom:1px solid #dbe4e7\"\u003eSample\u003c\/th\u003e\n\u003ctd style=\"vertical-align:top;padding:12px 0;border-bottom:1px solid #dbe4e7\"\u003eBacterial cells; manufacturer protocol uses 1-3 ml culture.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"text-align:left;vertical-align:top;padding:12px 16px 12px 0;width:32%;font-weight:600;border-bottom:1px solid #dbe4e7\"\u003eMethod and performance\u003c\/th\u003e\n\u003ctd style=\"vertical-align:top;padding:12px 0;border-bottom:1px solid #dbe4e7\"\u003eAlkaline lysis and binding columns; plasmids up to 10 kb; up to 20 µg DNA. pH indicator assists binding; centrifuge or vacuum-manifold workflows.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"text-align:left;vertical-align:top;padding:12px 16px 12px 0;width:32%;font-weight:600;border-bottom:1px solid #dbe4e7\"\u003ePack sizes\u003c\/th\u003e\n\u003ctd style=\"vertical-align:top;padding:12px 0;border-bottom:1px solid #dbe4e7\"\u003e50 preparations \u003cspan style=\"opacity:.7\"\u003e(PP-204S)\u003c\/span\u003e · 250 preparations \u003cspan style=\"opacity:.7\"\u003e(PP-204L)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"text-align:left;vertical-align:top;padding:12px 16px 12px 0;width:32%;font-weight:600;border-bottom:1px solid #dbe4e7\"\u003eStorage\u003c\/th\u003e\n\u003ctd style=\"vertical-align:top;padding:12px 0;border-bottom:1px solid #dbe4e7\"\u003eAmbient except RNase A at -20°C; neutralisation buffer containing RNase A at 4°C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"text-align:left;vertical-align:top;padding:12px 16px 12px 0;width:32%;font-weight:600;border-bottom:1px solid #dbe4e7\"\u003eShipping\u003c\/th\u003e\n\u003ctd style=\"vertical-align:top;padding:12px 0;border-bottom:1px solid #dbe4e7\"\u003eShipped at ambient temperature.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"text-align:left;vertical-align:top;padding:12px 16px 12px 0;width:32%;font-weight:600;border-bottom:1px solid #dbe4e7\"\u003eShelf life\u003c\/th\u003e\n\u003ctd style=\"vertical-align:top;padding:12px 0;border-bottom:1px solid #dbe4e7\"\u003e12 months under recommended storage; remaining shelf life depends on the supplied lot.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003ch3\u003eApplications\u003c\/h3\u003e\u003cp\u003eResearch amplification, sequencing, restriction digestion and transformation.\u003c\/p\u003e\n\u003ch3\u003eKit contents\u003c\/h3\u003e\u003cp\u003eLysis buffer with pH indicator, neutralisation, activation, washing and elution buffers; RNase A; binding columns and collection tubes.\u003c\/p\u003e\n\u003ch3\u003eYou will also need\u003c\/h3\u003e\u003cp\u003e96-99% ethanol and 1.5 ml tubes; suitable centrifuge or vacuum manifold.\u003c\/p\u003e\n\u003ch3\u003eDocuments\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.jenabioscience.com\/images\/MSDS\/PP-204_MSDS.0002.pdf\" target=\"_blank\" rel=\"noopener\"\u003eSafety data sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.jenabioscience.com\/images\/PDF\/PP-204.0002.pdf\" target=\"_blank\" rel=\"noopener\"\u003eDatasheet and protocol (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.jenabioscience.com\/molecular-biology\/rna-dna-preparation-cleanup\/plasmid-dna-column-kit\/pp-204-plasmid-mini-prep-kit-column-kit\" target=\"_blank\" rel=\"noopener\"\u003eManufacturer product page\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"font-size:.85em;opacity:.75;margin-top:20px\"\u003eSupplied by HAYU Biomedical Limited. Product image is representative. Please check specifications before ordering.\u003c\/p\u003e","brand":"Jena Bioscience","offers":[{"title":"50 preparations","offer_id":64777712075129,"sku":"PP-204S","price":53.79,"currency_code":"GBP","in_stock":true},{"title":"250 preparations","offer_id":64777712107897,"sku":"PP-204L","price":221.91,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0945\/3043\/7497\/files\/PP-204-1-white_2807a18b-bdf6-45e4-95c7-0c8fbf64501a.png?v=1791046848","url":"https:\/\/hayubiomedical.co.uk\/products\/jena-pp-204","provider":"HAYU Biomedical Limited","version":"1.0","type":"link"}