{"product_id":"jena-pp-214","title":"Bacteria DNA Preparation - Column Kit","description":"\u003cdiv style=\"border-left:3px solid #097e7d;background:#f2f8f8;padding:10px 14px;margin:0 0 18px;font-size:.92em\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO).\u003c\/strong\u003e Not for diagnostic or therapeutic use.\u003c\/div\u003e\n\u003cp style=\"font-size:1.06em;line-height:1.7\"\u003eSpin-column purification of bacterial genomic DNA.\u003c\/p\u003e\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\u003cdiv style=\"overflow-x:auto\"\u003e\u003ctable style=\"width:100%;border-collapse:collapse;margin:0 0 8px\"\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"text-align:left;vertical-align:top;padding:12px 16px 12px 0;width:32%;font-weight:600;border-bottom:1px solid #dbe4e7\"\u003eSample\u003c\/th\u003e\n\u003ctd style=\"vertical-align:top;padding:12px 0;border-bottom:1px solid #dbe4e7\"\u003eGram-positive and Gram-negative bacteria; generally up to 10^8 cells.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"text-align:left;vertical-align:top;padding:12px 16px 12px 0;width:32%;font-weight:600;border-bottom:1px solid #dbe4e7\"\u003eMethod and performance\u003c\/th\u003e\n\u003ctd style=\"vertical-align:top;padding:12px 0;border-bottom:1px solid #dbe4e7\"\u003eUp to 30 µg genomic DNA, 200 bp-50 kb. No phenol\/chloroform extraction.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"text-align:left;vertical-align:top;padding:12px 16px 12px 0;width:32%;font-weight:600;border-bottom:1px solid #dbe4e7\"\u003ePack sizes\u003c\/th\u003e\n\u003ctd style=\"vertical-align:top;padding:12px 0;border-bottom:1px solid #dbe4e7\"\u003e50 preparations \u003cspan style=\"opacity:.7\"\u003e(PP-214S)\u003c\/span\u003e · 250 preparations \u003cspan style=\"opacity:.7\"\u003e(PP-214L)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"text-align:left;vertical-align:top;padding:12px 16px 12px 0;width:32%;font-weight:600;border-bottom:1px solid #dbe4e7\"\u003eStorage\u003c\/th\u003e\n\u003ctd style=\"vertical-align:top;padding:12px 0;border-bottom:1px solid #dbe4e7\"\u003eAmbient kit; reconstituted RNase A, lysozyme and Proteinase K at -20°C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"text-align:left;vertical-align:top;padding:12px 16px 12px 0;width:32%;font-weight:600;border-bottom:1px solid #dbe4e7\"\u003eShipping\u003c\/th\u003e\n\u003ctd style=\"vertical-align:top;padding:12px 0;border-bottom:1px solid #dbe4e7\"\u003eShipped at ambient temperature.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"text-align:left;vertical-align:top;padding:12px 16px 12px 0;width:32%;font-weight:600;border-bottom:1px solid #dbe4e7\"\u003eShelf life\u003c\/th\u003e\n\u003ctd style=\"vertical-align:top;padding:12px 0;border-bottom:1px solid #dbe4e7\"\u003e12 months under recommended storage; remaining shelf life depends on the supplied lot.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003ch3\u003eApplications\u003c\/h3\u003e\u003cp\u003eResearch PCR\/qPCR, genotyping, Southern blotting and SNP\/SSR studies.\u003c\/p\u003e\n\u003ch3\u003eKit contents\u003c\/h3\u003e\u003cp\u003eResuspension, lysis, binding, activation, washing and elution buffers; RNase A; lysozyme; Proteinase K; columns and 2 ml collection tubes.\u003c\/p\u003e\n\u003ch3\u003eYou will also need\u003c\/h3\u003e\u003cp\u003e96-99% ethanol, distilled water, 1.5\/2 ml tubes and suitable centrifugation\/incubation equipment.\u003c\/p\u003e\n\u003ch3\u003eDocuments\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.jenabioscience.com\/images\/MSDS\/PP-214_MSDS.0002.pdf\" target=\"_blank\" rel=\"noopener\"\u003eSafety data sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.jenabioscience.com\/images\/PDF\/PP-214.0003.pdf\" target=\"_blank\" rel=\"noopener\"\u003eDatasheet and protocol (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.jenabioscience.com\/molecular-biology\/rna-dna-preparation-cleanup\/genomic-dna-column-kits\/pp-214-bacteria-dna-preparation-column-kit\" target=\"_blank\" rel=\"noopener\"\u003eManufacturer product page\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"font-size:.85em;opacity:.75;margin-top:20px\"\u003eSupplied by HAYU Biomedical Limited. Product image is representative. Please check specifications before ordering.\u003c\/p\u003e","brand":"Jena Bioscience","offers":[{"title":"50 preparations","offer_id":64777711485305,"sku":"PP-214S","price":84.44,"currency_code":"GBP","in_stock":true},{"title":"250 preparations","offer_id":64777711518073,"sku":"PP-214L","price":367.54,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0945\/3043\/7497\/files\/PP-214-1-white_e0e820f8-bb3c-4c6c-819f-02c83a72bdcb.png?v=1791047351","url":"https:\/\/hayubiomedical.co.uk\/products\/jena-pp-214","provider":"HAYU Biomedical Limited","version":"1.0","type":"link"}