{"product_id":"jena-pp-218","title":"SAP-Exo Kit","description":"\u003cdiv style=\"border-left:3px solid #097e7d;background:#f2f8f8;padding:10px 14px;margin:0 0 18px;font-size:.92em\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO).\u003c\/strong\u003e Not for diagnostic or therapeutic use.\u003c\/div\u003e\n\u003cp style=\"font-size:1.06em;line-height:1.7\"\u003eEnzymatic cleanup of PCR products using shrimp alkaline phosphatase and exonuclease I. The protocol uses 2 µl reagent per 5 µl PCR product.\u003c\/p\u003e\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\u003cdiv style=\"overflow-x:auto\"\u003e\u003ctable style=\"width:100%;border-collapse:collapse;margin:0 0 8px\"\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"text-align:left;vertical-align:top;padding:12px 16px 12px 0;width:32%;font-weight:600;border-bottom:1px solid #dbe4e7\"\u003eSample\u003c\/th\u003e\n\u003ctd style=\"vertical-align:top;padding:12px 0;border-bottom:1px solid #dbe4e7\"\u003ePCR products.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"text-align:left;vertical-align:top;padding:12px 16px 12px 0;width:32%;font-weight:600;border-bottom:1px solid #dbe4e7\"\u003eMethod and performance\u003c\/th\u003e\n\u003ctd style=\"vertical-align:top;padding:12px 0;border-bottom:1px solid #dbe4e7\"\u003eRecombinant shrimp alkaline phosphatase plus exonuclease I remove residual dNTPs and primers. 20-minute treatment: 10 minutes at 37°C, then 10 minutes at 80°C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"text-align:left;vertical-align:top;padding:12px 16px 12px 0;width:32%;font-weight:600;border-bottom:1px solid #dbe4e7\"\u003ePack sizes\u003c\/th\u003e\n\u003ctd style=\"vertical-align:top;padding:12px 0;border-bottom:1px solid #dbe4e7\"\u003e500 µl \u003cspan style=\"opacity:.7\"\u003e(PP-218S)\u003c\/span\u003e · 5 x 500 µl \u003cspan style=\"opacity:.7\"\u003e(PP-218L)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"text-align:left;vertical-align:top;padding:12px 16px 12px 0;width:32%;font-weight:600;border-bottom:1px solid #dbe4e7\"\u003eStorage\u003c\/th\u003e\n\u003ctd style=\"vertical-align:top;padding:12px 0;border-bottom:1px solid #dbe4e7\"\u003e-20°C; avoid repeated freeze\/thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"text-align:left;vertical-align:top;padding:12px 16px 12px 0;width:32%;font-weight:600;border-bottom:1px solid #dbe4e7\"\u003eShipping\u003c\/th\u003e\n\u003ctd style=\"vertical-align:top;padding:12px 0;border-bottom:1px solid #dbe4e7\"\u003eShipped on gel packs.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"text-align:left;vertical-align:top;padding:12px 16px 12px 0;width:32%;font-weight:600;border-bottom:1px solid #dbe4e7\"\u003eShelf life\u003c\/th\u003e\n\u003ctd style=\"vertical-align:top;padding:12px 0;border-bottom:1px solid #dbe4e7\"\u003e12 months under recommended storage; remaining shelf life depends on the supplied lot.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003ch3\u003eApplications\u003c\/h3\u003e\u003cp\u003eResearch sequencing, genotyping, cloning and SNP workflows.\u003c\/p\u003e\n\u003ch3\u003eWhat’s supplied\u003c\/h3\u003e\u003cp\u003eSAP-Exo Mix, red cap. Use 2 µl mix per 5 µl PCR product; 500 µl provides 250 standard treatments and 5 × 500 µl provides 1,250.\u003c\/p\u003e\n\u003ch3\u003eYou will also need\u003c\/h3\u003e\u003cp\u003ePCR samples, pipetting equipment and temperature-controlled incubation.\u003c\/p\u003e\n\u003ch3\u003eDocuments\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.jenabioscience.com\/images\/MSDS\/PP-218_MSDS.0003.pdf\" target=\"_blank\" rel=\"noopener\"\u003eSafety data sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.jenabioscience.com\/images\/PDF\/PP-218.0002.pdf\" target=\"_blank\" rel=\"noopener\"\u003eDatasheet and protocol (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.jenabioscience.com\/molecular-biology\/rna-dna-preparation-cleanup\/dna-cleanup\/pp-218-sap-exo-kit\" target=\"_blank\" rel=\"noopener\"\u003eManufacturer product page\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"font-size:.85em;opacity:.75;margin-top:20px\"\u003eSupplied by HAYU Biomedical Limited. Product image is representative. Please check specifications before ordering.\u003c\/p\u003e","brand":"Jena Bioscience","offers":[{"title":"500 µl","offer_id":64777712271737,"sku":"PP-218S","price":147.99,"currency_code":"GBP","in_stock":true},{"title":"5 x 500 µl","offer_id":64777712304505,"sku":"PP-218L","price":591.8,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0945\/3043\/7497\/files\/PP-218-1-white_7e30e9f3-a8cb-4b73-80ea-83544becbaea.png?v=1791046685","url":"https:\/\/hayubiomedical.co.uk\/products\/jena-pp-218","provider":"HAYU Biomedical Limited","version":"1.0","type":"link"}