{"product_id":"kao-michayluk-medium-k0214","title":"KAO \u0026 Michayluk medium","description":"\u003cp\u003e\u003cstrong\u003eKAO \u0026amp; Michayluk medium\u003c\/strong\u003e by Duchefa Biochemie is supplied for plant tissue culture and laboratory research.\u003c\/p\u003e\u003ch3\u003eDescription and application\u003c\/h3\u003e\u003cp\u003eThe medium defined by Kao and Michayluk was designed to grow cells and protoplasts of Vicia hajastana at a very low population -density in liquid media. The inability of the plant cells to grow at a very low population density may be caused by excessive diffusion of metabolic intermediates into the medium, resulting in their dilution in the cell to a level below that required for survival. Vicia cells were able to grow at an initial population density of 1-2 cells\/ml when the mineral salt medium was enriched with organic acids, sugars, sugar alcohols, growth regulators, amino acids and other organic compounds. The percentage of cell division could be increased by raising the -concentration of CaCl2 from 1 mM, as in Gamborg B5, to 5 mM. Calcium may play an important role in the proces of cell division because of its ability to preserve the structural and functional integrity of plant cell membranes. The medium defined by Kao and Michayluk was designed to grow cells and protoplasts of Vicia hajastana at a very low population -density in liquid media. The inability of the plant cells to grow at a very low population density may be caused by excessive diffusion of metabolic intermediates into the medium, resulting in their dilution in the cell to a level below that required for survival. Vicia cells were able to grow at an initial population density of 1-2 cells\/ml when the mineral salt medium was enriched with organic acids, sugars, sugar alcohols, growth regulators, amino acids and other organic compounds. The percentage of cell division could be increased by raising the -concentration of CaCl2 from 1 mM, as in Gamborg B5, to 5 mM. Calcium may play an important role in the proces of cell division because of its ability to preserve the structural and functional integrity of plant cell membranes.\u003c\/p\u003e\u003ch3\u003eComposition and technical specifications\u003c\/h3\u003e\u003ctable class=\"table table-hover\"\u003e\n\t\t\t\t\t\t\u003cthead\u003e\n\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\u003cth class=\"col-sm-6\"\u003eMicro Elements\u003c\/th\u003e\n\t\t\t\t\t\t\t\t\u003cth class=\"col-sm-3\"\u003emg\/l\u003c\/th\u003e\n\t\t\t\t\t\t\t\t\u003cth class=\"col-sm-3\"\u003eµM\u003c\/th\u003e\n\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\u003c\/thead\u003e\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\u003ctbody\u003e\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eCoCl\u003csub\u003e2\u003c\/sub\u003e.6H\u003csub\u003e2\u003c\/sub\u003eO\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e0.025\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e0.11\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eCuSO\u003csub\u003e4\u003c\/sub\u003e.5H\u003csub\u003e2\u003c\/sub\u003eO\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e0.025\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e0.10\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eFeNaEDTA\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e36.70\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e100.00\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eH\u003csub\u003e3\u003c\/sub\u003eBO\u003csub\u003e3\u003c\/sub\u003e\n\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e3.00\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e48.52\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eKI\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e0.75\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e4.52\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eMnSO\u003csub\u003e4\u003c\/sub\u003e.H\u003csub\u003e2\u003c\/sub\u003eO\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e10.00\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e59.17\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eNa\u003csub\u003e2\u003c\/sub\u003eMoO\u003csub\u003e4\u003c\/sub\u003e.2H\u003csub\u003e2\u003c\/sub\u003eO\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e0.25\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e1.03\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eZnSO\u003csub\u003e4\u003c\/sub\u003e.7H\u003csub\u003e2\u003c\/sub\u003eO\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e2.00\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e6.96\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\u003c\/tbody\u003e\n\t\t\t\t\t\u003c\/table\u003e\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\u003ctable class=\"table table-hover\"\u003e\n\t\t\t\t\t\t\u003cthead\u003e\n\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\u003cth class=\"col-sm-6\"\u003eMacro Elements\u003c\/th\u003e\n\t\t\t\t\t\t\t\t\u003cth class=\"col-sm-3\"\u003emg\/l\u003c\/th\u003e\n\t\t\t\t\t\t\t\t\u003cth class=\"col-sm-3\"\u003emM\u003c\/th\u003e\n\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\u003c\/thead\u003e\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\u003ctbody\u003e\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eCaCl\u003csub\u003e2\u003c\/sub\u003e\n\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e453.00\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e4.08\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eKCl\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e300.00\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e4.02\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eKH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e\n\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e170.00\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e1.25\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eKNO\u003csub\u003e3\u003c\/sub\u003e\n\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e1900.00\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e18.79\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eMgSO\u003csub\u003e4\u003c\/sub\u003e\n\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e146.84\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e1.22\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ctr\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-6\"\u003eNH\u003csub\u003e4\u003c\/sub\u003eNO\u003csub\u003e3\u003c\/sub\u003e\n\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e600.00\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\t\u003ctd class=\"col-sm-3\"\u003e7.50\u003c\/td\u003e\n\t\t\t\t\t\t\t\t\u003c\/tr\u003e\n\t\t\t\t\t\t\t\t\t\t\t\t\t\u003c\/tbody\u003e\n\t\t\t\t\t\u003c\/table\u003e\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\u003cp\u003eKao K.N., O.L. Gamborg et al., The effects of sugars and inorganic salts on cell regeneration and sustained division in plant proto-plasts. Colloques internationaux C.N.R.S., 212, Protoplastes et fusion de cellules somatiques végétales.Kao K.N. and Michayluk M.R., Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta (Berl.), 126, 105 (1975).\u003c\/p\u003e\n\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ch4\u003eHazard statements\u003c\/h4\u003e\n\u003cul class=\"details\"\u003e\n\t\u003cli\u003e\n\u003cspan class=\"label label-warning\"\u003eH315\u003c\/span\u003e  Causes skin irritation\n\u003c\/li\u003e\n\t\u003cli\u003e\n\u003cspan class=\"label label-warning\"\u003eH319\u003c\/span\u003e  Causes serious eye irritation\n\u003c\/li\u003e\n\t\u003cli\u003e\n\u003cspan class=\"label label-warning\"\u003eH335\u003c\/span\u003e  May cause respiratory irritation\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\u003ch4\u003ePrecautionary statements\u003c\/h4\u003e\n\u003cul class=\"details\"\u003e\n\t\u003cli\u003e\n\u003cspan class=\"label label-warning\"\u003eP261\u003c\/span\u003e  Avoid breathing dust\/fume\/gas\/mist\/vapours\/spray\n\u003c\/li\u003e\n\t\u003cli\u003e\n\u003cspan class=\"label label-warning\"\u003eP302\u003c\/span\u003e  IF ON SKIN:\n\u003c\/li\u003e\n\t\u003cli\u003e\n\u003cspan class=\"label label-warning\"\u003eP305\u003c\/span\u003e  IF IN EYES:\n\u003c\/li\u003e\n\t\u003cli\u003e\n\u003cspan class=\"label label-warning\"\u003eP351\u003c\/span\u003e  Rinse continuously with water for several minutes\n\u003c\/li\u003e\n\t\u003cli\u003e\n\u003cspan class=\"label label-warning\"\u003eP352\u003c\/span\u003e  Wash with soap and water\n\u003c\/li\u003e\n\u003c\/ul\u003e\t\n\t\t\t\t\t\t\t\u003cp\u003e\u003cstrong\u003eAvailable pack options:\u003c\/strong\u003e 5 l, 10 l. \u003cstrong\u003eManufacturer reference:\u003c\/strong\u003e K0214.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/duchefa-biochemie.com\/msds\/download\/number\/K0214\/name\/kao-michayluk-medium\/id\/1973\"\u003eSafety data sheet\u003c\/a\u003e\u003c\/p\u003e","brand":"Duchefa Biochemie","offers":[{"title":"5 l","offer_id":64645426020729,"sku":"K0214.0005","price":11.95,"currency_code":"GBP","in_stock":true},{"title":"10 l","offer_id":64645426053497,"sku":"K0214.0010","price":18.95,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0945\/3043\/7497\/files\/K0214_37f748bb-9b31-459b-9eec-f059f9692fe7.png?v=1789839716","url":"https:\/\/hayubiomedical.co.uk\/products\/kao-michayluk-medium-k0214","provider":"HAYU Biomedical Limited","version":"1.0","type":"link"}