{"product_id":"redsafe™-nucleic-acid-staining-solution","title":"RedSafe™ Nucleic Acid Staining Solution","description":"\u003cp\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRedSafe™ Nucleic Acid Staining Solution\u003c\/strong\u003e from iNtRON Biotechnology is a 20,000X concentrated fluorescent stain for visualisation of DNA and RNA during agarose gel electrophoresis.\u003c\/p\u003e\n\u003cp\u003eRedSafe™ is designed as a safer alternative to traditional ethidium bromide staining while providing comparable nucleic acid detection sensitivity. The stain emits green fluorescence when bound to DNA or RNA and can be incorporated directly into the agarose gel before casting.\u003c\/p\u003e\n\u003cp\u003eWhen bound to nucleic acids, RedSafe™ has fluorescence excitation maxima centred at approximately 309 nm and 419 nm, together with an additional visible excitation at approximately 514 nm. Its fluorescence emission maximum is centred at approximately 537 nm.\u003c\/p\u003e\n\u003cp\u003eThe stain is suitable for detecting double-stranded DNA and single-stranded RNA in agarose gels. Manufacturer testing shows detection of PCR products, genomic DNA, DNA markers and total RNA under UV illumination.\u003c\/p\u003e\n\u003cp\u003eThe standard pre-staining procedure uses 5 µL of RedSafe™ 20,000X concentrate per 100 mL of prepared agarose solution. The stain is mixed into the molten agarose before the gel is cast, eliminating the need for a separate post-electrophoresis staining step.\u003c\/p\u003e\n\u003cp\u003eRedSafe™ can be used for routine PCR product analysis, restriction-fragment analysis, plasmid DNA analysis, genomic DNA analysis, RNA analysis and isolation of DNA fragments for downstream cloning workflows.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e 20,000X concentrated nucleic acid stain • Detects DNA and RNA • Emits green fluorescence when bound to nucleic acids • Comparable sensitivity to ethidium bromide • Suitable for pre-cast agarose gel staining • Simple addition directly to molten agarose • Compatible with UV detection • Visible excitation at approximately 514 nm • Suitable for DNA fragment recovery and downstream cloning • Long-term stability under recommended storage conditions\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSample Types:\u003c\/strong\u003e PCR Products • DNA Markers • Genomic DNA • Plasmid DNA • Restriction Fragments • Double-Stranded DNA • Single-Stranded RNA • Total RNA • Other Compatible Nucleic Acid Samples\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e Agarose Gel Electrophoresis • PCR Product Visualisation • DNA Fragment Detection • DNA Size Analysis • Genomic DNA Analysis • Plasmid DNA Analysis • Restriction Fragment Analysis • RNA Visualisation • Gel Documentation • DNA Fragment Isolation for Subcloning\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResearch Use:\u003c\/strong\u003e Research Use Only (RUO)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eManufacturer:\u003c\/strong\u003e iNtRON Biotechnology\u003c\/p\u003e\n\u003ch3 style=\"margin-top: 22px; margin-bottom: 10px;\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv style=\"overflow-x: auto; width: 100%; margin-bottom: 24px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 14px; line-height: 1.4;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"border: 1px solid #d9d9d9; background: #f5f5f5; padding: 9px 10px; text-align: left; width: 42%;\"\u003eSpecification\u003c\/th\u003e\n\u003cth style=\"border: 1px solid #d9d9d9; background: #f5f5f5; padding: 9px 10px; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eRedSafe™ Nucleic Acid Staining Solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eCatalogue No.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e21141\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003ePack Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e20,000X concentrate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eTarget Molecules\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eDouble-stranded DNA and single-stranded RNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eFluorescence Colour\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eGreen\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eExcitation Maximum 1\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eApproximately 309 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eExcitation Maximum 2\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eApproximately 419 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eVisible Excitation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eApproximately 514 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eEmission Maximum\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eApproximately 537 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eRecommended Working Dilution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e1:20,000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eStandard Usage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e5 µL per 100 mL molten agarose solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eRecommended Agarose Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eApproximately 0.8–3%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eRecommended Maximum Gel Thickness\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eLess than 0.5 cm for optimal sensitivity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eDetection Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eUV illumination; compatible visible excitation may also be used with a suitable imaging system\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eRelative Sensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eComparable to ethidium bromide under recommended conditions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eRoom-Temperature Stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eMore than 12 months under recommended conditions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eRecommended Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e2–8°C for improved long-term stability\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eClassification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eResearch Use Only (RUO)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003e\u003cstrong\u003eManufacturer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #d9d9d9; padding: 8px 10px;\"\u003eiNtRON Biotechnology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top: 22px; margin-bottom: 10px;\"\u003eRecommended Pre-Staining Procedure\u003c\/h3\u003e\n\u003col style=\"padding-left: 22px; margin-bottom: 20px;\"\u003e\n\u003cli style=\"margin-bottom: 7px;\"\u003ePrepare 100 mL of 0.8–3% agarose solution in the appropriate electrophoresis buffer.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 7px;\"\u003eHeat until the agarose is completely dissolved and the solution is clear.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 7px;\"\u003eAdd 5 µL of RedSafe™ 20,000X to the 100 mL agarose solution.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 7px;\"\u003eSwirl gently to mix while avoiding the formation of bubbles.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 7px;\"\u003ePour the agarose into the prepared casting tray and insert the comb.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 7px;\"\u003eAllow the gel to solidify, load the samples and perform electrophoresis.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 7px;\"\u003eVisualise the nucleic acid bands using a compatible UV or fluorescence imaging system.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp style=\"font-size: 13px; color: #666;\"\u003e\u003cem\u003eImportant: Repeated melting of agarose gels containing RedSafe™ may reduce staining sensitivity. For optimal detection, the manufacturer recommends keeping the gel thickness below 0.5 cm.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp style=\"font-size: 13px; color: #666;\"\u003e\u003cem\u003eSafety: Although RedSafe™ is positioned as a safer alternative to ethidium bromide, it may cause skin or eye irritation. Wear suitable gloves and eye protection, consult the safety data sheet, and dispose of the stain and stained gels according to applicable institutional and local requirements.\u003c\/em\u003e\u003c\/p\u003e","brand":"iNtRON Biotechnology","offers":[{"title":"1 mL","offer_id":64326478004601,"sku":"21141","price":58.45,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0945\/3043\/7497\/files\/ChatGPTImageAug21_2026_04_15_44PM.png?v=1787325390","url":"https:\/\/hayubiomedical.co.uk\/products\/redsafe%e2%84%a2-nucleic-acid-staining-solution","provider":"HAYU Biomedical Limited","version":"1.0","type":"link"}