{"product_id":"smo-hifi-dna-polymerase-tf1000","title":"SMO-HiFi™ DNA Polymerase, 1 U\/µL, 100 U","description":"\u003cp\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eSMO-HiFi™ DNA Polymerase\u003c\/strong\u003e is a genetically modified recombinant polymerase designed for high-fidelity and rapid PCR amplification. Its 3′→5′ exonuclease activity provides proofreading and approximately 70-fold higher fidelity than conventional Taq polymerase.\u003c\/p\u003e\n\n\u003cp\u003eThe enzyme can amplify suitable DNA targets up to 12 kb and generates blunt-ended PCR products. It is supplied with HiFi buffer, MgSO₄, dNTP mix and DMSO for flexible reaction optimisation.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e Approximately 70-fold higher fidelity than Taq • 3′→5′ proofreading activity • Fast DNA synthesis • Amplification up to 12 kb • Blunt-ended PCR products • High thermostability • Sensitivity down to 1 fg template DNA • Complete supporting reagent set\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e High-Fidelity PCR • Long-Range PCR • Gene Cloning • Blunt-End Cloning • DNA Sequencing Template Preparation • Site-Directed Mutagenesis • Genomic DNA Amplification • cDNA Amplification\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Suitable for PCR applications requiring accurate DNA synthesis and blunt-ended products. Magnesium concentration, DMSO and cycling conditions should be optimised for the template and primer system.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003ePackaging:\u003c\/strong\u003e 100 µL enzyme at 1 U\/µL, providing 100 units and approximately 100 standard reactions when using 1 unit per reaction. Supporting buffer, MgSO₄, dNTPs and DMSO are included.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eManufacturer:\u003c\/strong\u003e SMOBIO Technology\u003c\/p\u003e\n\n\u003ch3 style=\"margin-top:22px; margin-bottom:10px;\"\u003eTechnical Specifications\u003c\/h3\u003e\n\n\u003cdiv style=\"overflow-x:auto; width:100%; margin-bottom:24px;\"\u003e\n\u003ctable style=\"width:100%; border-collapse:collapse; font-size:14px; line-height:1.4;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"border:1px solid #d9d9d9; background:#f5f5f5; padding:9px 10px; text-align:left; width:42%;\"\u003eSpecification\u003c\/th\u003e\n\u003cth style=\"border:1px solid #d9d9d9; background:#f5f5f5; padding:9px 10px; text-align:left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eTF1000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eSMO-HiFi™ DNA Polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eHigh-fidelity DNA polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eEnzyme Source\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eGenetically modified recombinant DNA polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eEnzyme Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e1 U\/µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eTotal Enzyme Quantity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e100 U\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eEnzyme Volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e100 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003e5′→3′ DNA Polymerase Activity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eYes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003e3′→5′ Exonuclease Activity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eYes; proofreading activity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eRelative Fidelity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eApproximately 70 times higher than Taq DNA polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003ePCR Product Ends\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eBlunt ends\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eMaximum Demonstrated Target\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eUp to 12 kb from λDNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eMaximum DNA Synthesis Rate\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eUp to approximately 1 kb per 10 seconds\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eProtocol Extension Time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e30 seconds per kb at 68°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eTemplate Sensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eAmplification demonstrated from as little as 1 fg DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eThermostability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eHalf-life greater than 10 hours at 95°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eTypical Reaction Volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e50 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eRecommended Enzyme per Reaction\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e1 U\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eRecommended Template Amount\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e1–150 ng per 50 µL reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eRecommended Primer Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e0.1–0.5 µM each\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eRecommended Extension Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e68°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003e10X HiFi™ Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e600 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eMgSO₄ Solution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e25 mM, 500 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003edNTP Mix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e2 mM each, 600 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eDMSO\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e600 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eApproximate Reaction Capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e100 reactions at 1 U per reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eEnzyme Storage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e50 mM Tris-HCl, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabiliser and 50% glycerol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eAmount incorporating 10 nmol dNTP into acid-insoluble material in 30 minutes at 74°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eStorage Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e−20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eStated Stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e24 months at −20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eManufacturer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eSMOBIO Technology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003e\u003cstrong\u003eClassification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #d9d9d9; padding:8px 10px;\"\u003eResearch Use Only (RUO)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\n\u003cp style=\"font-size:13px; color:#666;\"\u003e\u003cem\u003eNote: The 12 kb amplification result was demonstrated using λDNA and is template-dependent. Target length, fidelity and yield may vary with template quality, GC content, primer design and reaction conditions.\u003c\/em\u003e\u003c\/p\u003e","brand":"SMOBIO","offers":[{"title":"Default Title","offer_id":56871434584441,"sku":"TF1000","price":99.99,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0945\/3043\/7497\/files\/ChatGPTImageJun15_2026_04_23_42PM.png?v=1781537059","url":"https:\/\/hayubiomedical.co.uk\/products\/smo-hifi-dna-polymerase-tf1000","provider":"HAYU Biomedical Limited","version":"1.0","type":"link"}