PRODUCT INFORMATION
50X TAE Buffer
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PRODUCT INFORMATION
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01 Description, applications & specifications
Description
50X TAE Buffer is a concentrated Tris-acetate-EDTA running buffer for agarose gel electrophoresis of DNA and other nucleic acids.
Dilute the solution 1:50 with molecular biology-grade water to prepare 1X TAE. TAE supports relatively fast migration of linear double-stranded DNA and is particularly suitable for routine, low-voltage electrophoresis.
Key Features: 50X concentrated stock • Produces 1X TAE after dilution • Ready-to-dilute liquid • Supports DNA migration and stable conductivity • Suitable for agarose gel electrophoresis • Particularly useful for DNA fragments larger than 4 kb • Supports resolution of supercoiled DNA
Applications: Agarose Gel Electrophoresis • DNA Fragment Separation • PCR Product Analysis • Restriction Digest Analysis • Plasmid DNA Analysis • Nucleic Acid Electrophoresis • Molecular Biology Research
Compatibility: Suitable for horizontal agarose gel electrophoresis systems. TAE has a lower buffering capacity than TBE and may require buffer replacement or recirculation during extended or high-current electrophoresis runs.
Preparation: To prepare 1 L of 1X TAE, combine 20 mL of 50X TAE Buffer with 980 mL of molecular biology-grade water. To prepare 500 mL, combine 10 mL of 50X TAE with 490 mL of water.
Packaging: Available as ML016-100ML, ML016-500ML and ML016-2X500ML.
Manufacturer: HiMedia Laboratories
Technical Specifications
| Specification | Details |
|---|---|
| Base Product Code | ML016 |
| 100 mL SKU | ML016-100ML |
| 500 mL SKU | ML016-500ML |
| 2 × 500 mL SKU | ML016-2X500ML |
| Product Name | 50X TAE Buffer |
| Buffer Type | Tris-acetate-EDTA electrophoresis buffer |
| Concentration | 50X concentrate |
| Physical Form | Liquid |
| Principal Components | Tris, acetate and EDTA |
| Typical 50X Composition | 2 M Tris, 1 M acetate and 50 mM EDTA |
| 1X Working Composition | 40 mM Tris, 20 mM acetate and 1 mM EDTA |
| Dilution | 1 part 50X buffer with 49 parts molecular biology-grade water |
| Working Concentration | 1X |
| Primary Application | Agarose gel electrophoresis of nucleic acids |
| Recommended Electrophoresis | Routine and low-voltage DNA electrophoresis |
| DNA Migration | Linear double-stranded DNA generally migrates faster in TAE than in TBE |
| Buffering Capacity | Lower than TBE buffer |
| Recommended Storage | Room temperature, 15–25°C |
| Manufacturer | HiMedia Laboratories |
Note: Use freshly prepared 1X TAE for consistent electrophoresis results. Because TAE has a relatively low buffering capacity, avoid prolonged high-current runs unless the buffer is replaced or recirculated.
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