PRODUCT INFORMATION
AccuBand™ 50 bp DNA Ladder II, 50 bp–1 kb, 500 µL
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PRODUCT INFORMATION
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01 Description, applications & specifications
Description
AccuBand™ 50 bp DNA Ladder II is a ready-to-use DNA size marker containing 13 fragments from 50 to 1,000 bp. Enhanced 250 bp and 500 bp bands provide convenient reference points for DNA fragment identification.
The ladder provides clear low-molecular-weight bands and is suitable for both agarose gel and polyacrylamide gel electrophoresis. It is premixed with Orange G loading dye for direct gel loading.
Key Features: 50–1,000 bp range • 13 DNA fragments • Enhanced 250 bp and 500 bp bands • Clear low-molecular-weight bands • Agarose and DNA PAGE compatible • Ready to use • Premixed loading dye • Direct gel loading
Applications: DNA Fragment Sizing • Short PCR Product Analysis • Restriction Digest Analysis • Agarose Gel Electrophoresis • DNA Polyacrylamide Gel Electrophoresis • Molecular Biology Research
Compatibility: Suitable for agarose gel and native DNA PAGE. The ladder is not supplied in denatured form and must be separately denatured before use in denaturing polyacrylamide/urea gels.
Packaging: Supplied as 500 µL, providing approximately 100 applications at the recommended loading volume of 5 µL per well.
Manufacturer: SMOBIO Technology
Technical Specifications
| Specification | Details |
|---|---|
| SKU | DM1200 |
| Product Name | AccuBand™ 50 bp DNA Ladder II |
| Series | AccuBand™ |
| Product Type | DNA ladder |
| Size Range | 50–1,000 bp |
| Number of Bands | 13 |
| Fragment Sizes | 1,000, 900, 800, 700, 600, 500, 400, 300, 250, 200, 150, 100 and 50 bp |
| Enhanced Bands | 500 bp and 250 bp |
| DNA Concentration | 55.5 µg per 500 µL |
| Pack Size | 500 µL |
| Recommended Loading Volume | 5 µL per well |
| Approximate Number of Loads | 100 |
| Format | Ready to use; premixed with loading dye |
| Tracking Dye | Orange G |
| Approximate Tracking Position | Approximately 50 bp dsDNA |
| Fluorescent DNA Dye Included | No |
| Agarose Gel Compatibility | Yes |
| Native DNA PAGE Compatibility | Yes |
| Denaturing PAGE Format | Not pre-denatured; denaturation is required before use |
| Source | PCR products and specifically restriction-digested double-stranded DNA |
| Buffer | 10 mM Tris-HCl, pH 8.0, and 10 mM EDTA |
| Room-Temperature Stability | 6 months |
| Stability at 4°C | 12 months |
| Stability at −20°C | 36 months |
| Manufacturer | SMOBIO Technology |
| Classification | Research Use Only (RUO) |
Note: A compatible nucleic acid stain is required for band visualisation. The ladder is suitable for native DNA PAGE but must be denatured separately before use in denaturing polyacrylamide/urea gels.
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