Agarose LE – Molecular Biology Grade

Description

Agarose LE – Molecular Biology Grade from iNtRON Biotechnology is a high-purity agarose powder designed for routine analytical and preparative nucleic acid gel electrophoresis.

The agarose forms clear, mechanically stable gels for separation and analysis of DNA fragments. It is suitable for resolving DNA fragments ranging from approximately 500 bp to 23 kb, depending on the agarose concentration, electrophoresis buffer, running conditions and DNA conformation.

Agarose LE has low electroendosmosis characteristics, supporting efficient electrophoretic migration and reducing counterflow that can contribute to distorted or poorly resolved DNA bands. Its high transparency and low staining background facilitate visualisation and documentation of nucleic acid fragments after electrophoresis.

The product is suitable for preparing gels with commonly used electrophoresis buffers such as TAE and TBE. Gel concentration can be adjusted according to the size of the DNA fragments being analysed: lower agarose concentrations provide improved separation of larger fragments, while higher concentrations provide better resolution of smaller fragments.

DNase and RNase activity are reported as not detected, supporting use in molecular biology applications where nucleic acid integrity is important.

Key Features: High-purity molecular biology-grade agarose • Low electroendosmosis • Suitable for approximately 500 bp–23 kb DNA fragments • High gel clarity • Low staining background • Strong and easy-to-handle gels • Adjustable pore size through gel concentration • Compatible with routine TAE and TBE electrophoresis • DNase activity not detected • RNase activity not detected • Suitable for analytical and preparative electrophoresis

Sample Types: PCR Products • Restriction Digestion Products • Plasmid DNA • Genomic DNA Fragments • Cloning Products • Other DNA Samples

Applications: Agarose Gel Electrophoresis • PCR Product Analysis • Restriction Fragment Analysis • Plasmid DNA Analysis • DNA Size Determination • DNA Fragment Separation • Preparative Electrophoresis • DNA Gel Extraction Workflows • Southern Blotting • Routine Molecular Biology

Research Use: Research Use Only (RUO)

Manufacturer: iNtRON Biotechnology

Technical Specifications

General Gel Preparation Guide

Note: The gel concentrations above are general electrophoresis guidance. The optimal agarose concentration and running conditions should be selected according to the size and structure of the nucleic acid fragments, electrophoresis buffer, gel dimensions and intended downstream application.