SMOChem™ 1X MOPS-SDS Running Buffer Powder

Description

SMOChem™ 1X MOPS-SDS Running Buffer Powder is a convenient, premeasured electrophoresis buffer from SMOBIO Technology for denaturing protein separation by SDS-PAGE using the Bis-Tris buffer system.

The buffer is designed for use with Q-PAGE™ Bis-Tris Precast Gels and other compatible polyacrylamide gels operating with MOPS-SDS running buffer. It supports reproducible protein migration and effective separation of medium- and larger-sized proteins.

Compared with MES-SDS running buffer, MOPS-SDS generally provides improved separation of medium- to high-molecular-weight proteins. This makes it suitable for routine protein characterization, protein-purity assessment, recombinant protein analysis and sample separation before Western blotting.

Each package contains five individually measured powder pouches. One complete pouch is dissolved in deionized water to prepare 1 litre of ready-to-use 1X MOPS-SDS buffer, providing a total preparation volume of 5 litres per product pack.

The prepared 1X buffer contains 50 mM Tris base, 50 mM MOPS, 0.1% SDS and 1 mM EDTA. No pH adjustment is required. The premeasured powder dissolves within minutes, reducing weighing errors and improving consistency between electrophoresis runs.

Sodium dodecyl sulfate, or SDS, denatures proteins and provides a broadly uniform negative charge-to-mass ratio. Protein migration during electrophoresis therefore depends primarily on molecular size rather than intrinsic electrical charge.

Key Features: Premeasured powder formulation • Five individually packaged pouches • Each pouch prepares 1 litre of 1X buffer • Five-litre total preparation volume • No pH adjustment required • Dissolves within minutes • Optimized for Bis-Tris SDS-PAGE • Suitable for medium- and larger-sized proteins • Standardized composition • Reliable and reproducible protein separation • Compatible with Q-PAGE™ Bis-Tris Precast Gels • Room-temperature storage

Applications: Bis-Tris SDS-PAGE • Denaturing Protein Electrophoresis • Q-PAGE™ Bis-Tris Gel Electrophoresis • Medium- and High-Molecular-Weight Protein Separation • Protein Molecular-Weight Analysis • Protein-Purity Assessment • Recombinant Protein Analysis • Protein Expression Studies • Western Blot Sample Separation

Final 1X Composition: 50 mM Tris base • 50 mM MOPS • 0.1% SDS • 1 mM EDTA

Preparation: Empty the complete contents of one pouch into a suitable laboratory container. Add deionized water and dissolve the powder completely, then adjust the final volume to 1 litre with deionized water. No pH adjustment is required.

Important Preparation Note: Do not divide a powder pouch to prepare a smaller volume. Uneven distribution of the different powder components may alter the final buffer composition or pH. Always use one complete pouch to prepare 1 litre of 1X buffer.

Use Recommendation: Use fresh buffer for consistent electrophoresis performance. Reusing running buffer may alter its pH and ionic strength and may introduce residual protein contamination that affects migration and band resolution.

Storage: Store unopened powder pouches at room temperature. The manufacturer states a shelf life of 24 months under the recommended storage conditions.

Research Use: Research Use Only (RUO). Not intended for diagnostic or clinical use.

Manufacturer: SMOBIO Technology, Inc.

Technical Specifications

Important: Use MOPS-SDS buffer with compatible Bis-Tris gels. It should not be substituted with Tris-Glycine-SDS running buffer, as using an incompatible buffer system can alter protein migration and reduce separation quality.