SMOChem™ dUTP Solution – Sodium Salt (100 mM), 100 mL

Description

SMOChem™ dUTP Solution – Sodium Salt (100 mM), 100 mL is an ultrapure solution of 2′-deoxyuridine-5′-triphosphate supplied as the sodium salt in purified water. It is manufactured by SMOBIO Technology for PCR, RT-PCR and other molecular biology applications requiring a reliable source of dUTP.

dUTP can be incorporated into amplification products in place of dTTP. This enables the use of uracil-DNA glycosylase, also known as UNG or UDG, to degrade uracil-containing products from previous reactions before amplification begins. The dUTP/UNG system helps reduce false-positive results caused by carryover contamination while leaving natural thymine-containing DNA templates unaffected.

The solution has a nominal concentration of 100 mM, with a manufacturer specification of 100–110 mM. It is supplied in purified water at pH 8.5 ± 0.1 and has a purity of at least 99% as confirmed by HPLC.

The product is tested for protease, DNase, RNase and nicking activity. It is also tested for contamination with human DNA using an 18S rRNA target and bacterial DNA using a 23S rRNA target.

Key Features: Ultrapure dUTP sodium salt • 100 mM concentration • ≥99% purity by HPLC • Suitable for replacing dTTP in amplification reactions • Supports dUTP/UNG carryover-contamination control • Nuclease and ribonuclease free • Tested for human and bacterial DNA contamination • Stable for 36 months at −20°C • Suitable for PCR and RT-PCR research workflows

Applications: PCR • RT-PCR • Real-Time PCR • dUTP/UNG Carryover-Contamination Prevention • DNA Amplification • cDNA Amplification • Uracil-Containing DNA Synthesis • Molecular Biology Research

Formulation: 2′-Deoxyuridine-5′-triphosphate sodium salt at 100 mM in purified water, pH 8.5 ± 0.1.

Storage: Store at −20°C. The manufacturer states a shelf life of 36 months under the recommended storage conditions. To maintain product integrity, use appropriate aliquoting practices where repeated access or freeze–thaw cycles are expected.

Research Use: Research Use Only (RUO). Not intended for diagnostic, therapeutic or clinical use.

Manufacturer: SMOBIO Technology, Inc.

Technical Specifications

Note: Substitution of dTTP with dUTP may affect amplification efficiency depending on the polymerase and assay design. The dUTP-to-dTTP ratio, magnesium concentration and cycling conditions should be optimized for the selected PCR system.