CHU (N6) Medium

CHU (N6) Medium by Duchefa Biochemie is supplied for plant tissue culture and laboratory research.

Description and application

Chu (N6) medium is defined to improve the formation, growth and differentiation of pollen callus in rice. The concentration of ammonium proved to be crucial for the development of callus. The optimum -concentration NH4+ is 7.0 mM (equal to 3.5 mM (NH4)2SO4). Higher concentrations of ammonium drastically inhibited the growth and -differentiation of the rice pollen. The concentration of KNO3 and the other medi-um compo-nents did not affect the development of the -callus.Chu (N6) medium is defined to improve the formation, growth and differentiation of pollen callus in rice. The concentration of ammonium proved to be crucial for the development of callus. The optimum -concentration NH4+ is 7.0 mM (equal to 3.5 mM (NH4)2SO4). Higher concentrations of ammonium drastically inhibited the growth and -differentiation of the rice pollen. The concentration of KNO3 and the other medi-um compo-nents did not affect the development of the -callus.Chu (N6) medium is defined to improve the formation, growth and differentiation of pollen callus in rice. The concentration of ammonium proved to be crucial for the development of callus. The optimum -concentration NH4+ is 7.0 mM (equal to 3.5 mM (NH4)2SO4). Higher concentrations of ammonium drastically inhibited the growth and -differentiation of the rice pollen. The concentration of KNO3 and the other medi-um compo-nents did not affect the development of the -callus.

Composition and technical specifications

Micro Elements	mg/l	µM
FeNaEDTA	36.70	100.00
H3BO3	1.60	25.88
KI	0.80	4.81
MnSO4.H2O	3.33	19.70
ZnSO4.7H2O	1.50	5.22

Macro Elements	mg/l	mM
CaCl2	125.33	1.13
KH2PO4	400.00	2.94
KNO3	2830.00	27.99
MgSO4	90.27	0.75
(NH4)2SO4	463.00	3.50

Chu C.C, The N6 medium and its application to anther culture of -cereal crops, Proc. Symp. Plant Tissue Cult., Peking, 43 (1978).Chu C.C. et al., Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen -sources. Scienta Sinic., 18, 659 (1975).

Hazard statements

• H315 Causes skin irritation
• H319 Causes serious eye irritation
• H335 May cause respiratory irritation

Precautionary statements

• P261 Avoid breathing dust/fume/gas/mist/vapours/spray
• P302 IF ON SKIN:
• P305 IF IN EYES:
• P351 Rinse continuously with water for several minutes
• P352 Wash with soap and water

Available pack options: 10 l, 50 l. Manufacturer reference: C0203.

Safety data sheet