CLC / Ipomoea EP medium

CLC / Ipomoea EP medium by Duchefa Biochemie is supplied for plant tissue culture and laboratory research.

Description and application

Sweetpotato somatic embryo production is accomplished in two stages. Embryogenic callus is continuously proliferated by subculture on media containing 10 µM 2,4-D and 1 µM 6-BAP. Increa-sing the K+ concentration to 40-60 mM doubled the production of embryogenic callus, while the production of non embry-ogenic callus was reduced by 40%. The development of embryos, trigge-red by the removal of 2,4-D and 6-BAP, was enhan-ced by decrea-sing ammonium (NH4+) from 20 to 10 mM. Sweetpotato somatic embryo production is accomplished in two stages. Embryogenic callus is continuously proliferated by subculture on media containing 10 µM 2,4-D and 1 µM 6-BAP. Increa-sing the K+ concentration to 40-60 mM doubled the production of embryogenic callus, while the production of non embry-ogenic callus was reduced by 40%. The development of embryos, trigge-red by the removal of 2,4-D and 6-BAP, was enhan-ced by decrea-sing ammonium (NH4+) from 20 to 10 mM. Sweetpotato somatic embryo production is accomplished in two stages. Embryogenic callus is continuously proliferated by subculture on media containing 10 µM 2,4-D and 1 µM 6-BAP. Increa-sing the K+ concentration to 40-60 mM doubled the production of embryogenic callus, while the production of non embry-ogenic callus was reduced by 40%. The development of embryos, trigge-red by the removal of 2,4-D and 6-BAP, was enhan-ced by decrea-sing ammonium (NH4+) from 20 to 10 mM.

Composition and technical specifications

Micro Elements	mg/l	µM
CoCl2.6H2O	0.025
CuSO4.5H2O	0.025
FeNaEDTA	36.70
H3BO3	6.20
KI	0.83
MnSO4.H20	16.90
Na2MoO4.2H2O	0.25
ZnSO4.7H2O	8.60

Macro Elements	mg/l	mM
CaCl2	332.02
KH2PO4	170.00
KNO3	1900.00
MgSO4	180.54
NH4NO3	800.40

Vitamins	mg/l	µM
myo-Inositol	90.10
Nicotinic acid	1.23
Pyridoxine HCl	1.03
Thiamine HCl	1.69

Total concentration Micro and Macro elements (EP) including -vitamins: 3546.54 mg/l

Cheé R. et al., Optimizing Embryogenic Callus and Embryo Growth of a synthetic seed system for Sweetpotato by varying media nutrient concentrations. J. Am. Soc. Hort. Sci. 117, 663 (1992).

Hazard statements

• H315 Causes skin irritation
• H319 Causes serious eye irritation
• H335 May cause respiratory irritation

Precautionary statements

• P261 Avoid breathing dust/fume/gas/mist/vapours/spray
• P302 IF ON SKIN:
• P305 IF IN EYES:
• P351 Rinse continuously with water for several minutes
• P352 Wash with soap and water

Available pack options: 10 l, 50 l. Manufacturer reference: C0229.

Safety data sheet