De Greef & Jacobs medium including vitamins

De Greef & Jacobs medium including vitamins by Duchefa Biochemie is supplied for plant tissue culture and laboratory research.

Description and application

Callus derived from leaf pieces of sugarbeet, exposed to a cold period of 3-9 weeks, could be regenerated into a normal plant after being returned to normal temperature. On media free of growthregulators, a regenerating callus could be -formed with a high regeneration capacity.

Composition and technical specifications

Micro Elements	mg/l	µM
CoCl2.6H2O	0.0025	0.01
CuSO4.5H2O	0.0025	0.01
FeNaEDTA	36.70	100.00
H3BO3	10.62	171.76
KI	1.58	9.54
MnSO4.H2O	1.68	9.94
Na2MoO4.2H2O	0.0025	0.01
ZnSO4.7H2O	1.06	3.69

Macro Elements	mg/l	mM
CaCl2	226.50	2.04
KCl	600.00	8.05
KNO3	2000.00	19.78
MgSO4	244.33	2.03
NaH2PO4	250.00	2.08
(NH4)2SO4	400.00	3.03

Vitamins	mg/l	µM
myo-Inositol	100.00	554.94
Nicotinic acid	1.00	8.12
Pyridoxine HCl	1.00	4.86
Thiamine HCl	10.00	29.65

Total concentration Micro and Macro elements including -vitamins: 3882.44 mg/l

De Greef W. and Jacobs M. In vitro culture of the sugarbeet: Description of a cell line with high regeneration capacity., Plant Science Letters., 17, 55-61 (1979).

Hazard statements

• H315 Causes skin irritation
• H319 Causes serious eye irritation
• H335 May cause respiratory irritation

Precautionary statements

• P261 Avoid breathing dust/fume/gas/mist/vapours/spray
• P302 IF ON SKIN:
• P305 IF IN EYES:
• P351 Rinse continuously with water for several minutes
• P352 Wash with soap and water

Available pack options: 5 l, 50 l. Manufacturer reference: D0206.

Safety data sheet