DKW/Juglans medium including vitamins

DKW/Juglans medium including vitamins by Duchefa Biochemie is supplied for plant tissue culture and laboratory research.

Description and application

DKW medium has been defined for in vitro propagation of Paradox Walnut Rootstock (Juglans hindsii x J. regia) via nodal explants. The explants were placed on medium without growth-regulators for one week and subsequentely on medium containing 6-BAP and IBA. Opti-mum shoot development was supported under 4.5 µM 6-BAP and 5 nM IBA. The basal ends of the tissue culture derived shoots were dipped in 5 mM IBA solution and subsequen-tly rooted within 10 to 14 days in the greenhouse.

Composition and technical specifications

Micro Elements	mg/l	µM
CuSO4.5H2O	0.25	1.00
FeNaEDTA	44.63	121.61
H3BO3	4.80	77.63
MnSO4.H2O	33.80	200.00
Na2MoO4.2H2O	0.39	1.61
ZnSO4.7H2O	17.00	72.19

Macro Elements	mg/l	mM
CaCl2	112.50	1.01
Ca(NO3)2.2H2O	1664.64	8.30
KH2PO4	265.00	1.95
K2SO4	1559.00	8.95
MgSO4	361.49	3.00
NH4NO3	1416.00	17.70

Total concentration Micro and Macro elements: 5479.50 mg/l

Vitamins	mg/l	µM
Glycine	2.00	26.64
myo-Inositol	100.00	554.94
Nicotinic acid	1.00	8.12
Thiamine HCl	2.00	5.93

Total concentration Micro and Macro elements including -vitamins: 5584.50mg/l

river, J.A., Kuniyuki, A.H. In Vitro Propagation of Paradox walnut Rootstock, Hort. Science, 19(4), August 1984.

Hazard statements

• H315 Causes skin irritation
• H319 Causes serious eye irritation
• H335 May cause respiratory irritation

Precautionary statements

• P261 Avoid breathing dust/fume/gas/mist/vapours/spray
• P302 IF ON SKIN:
• P305 IF IN EYES:
• P351 Rinse continuously with water for several minutes
• P352 Wash with soap and water

Available pack options: 5 l, 10 l, 25 l, 50 l. Manufacturer reference: D0247.

Safety data sheet