DNA-maxi™ SV Plasmid DNA Purification Kit

Description

DNA-maxi™ SV Plasmid DNA Purification Kit from iNtRON Biotechnology provides an easy and efficient method for maxi-scale purification of high-quality plasmid DNA from bacterial cultures.

The kit combines an optimised alkaline lysis system with two specialised column types. A pre-filtration column removes precipitated cellular debris, proteins, genomic DNA and other insoluble material from the neutralised bacterial lysate. The clarified lysate is subsequently transferred to a silica-membrane binding column, where plasmid DNA selectively binds before washing and elution.

DNA-maxi™ SV supports both centrifugation-based spin processing and vacuum-manifold processing. The vacuum protocol can be completed in approximately 30 minutes, while the spin protocol takes approximately 80–90 minutes.

The kit is suitable for processing approximately 100–150 mL of bacterial culture containing a high-copy plasmid or up to 300–400 mL of culture containing a low-copy plasmid. Plasmids up to approximately 40 kb can be purified.

Manufacturer testing using plasmids ranging from 2.9 kb to approximately 40.2 kb demonstrated plasmid DNA recoveries of approximately 181–274 µg, depending on the plasmid and experimental conditions. Actual yield will vary according to culture volume, bacterial strain, plasmid copy number, plasmid size and culture conditions.

The resulting plasmid DNA is suitable for PCR, molecular cloning, automated or manual DNA sequencing, transfection, preparation of labelled hybridisation probes, electroporation, restriction enzyme digestion and other molecular biology applications.

Key Features: Maxi-scale plasmid DNA purification • Spin and vacuum protocols • Silica-membrane binding technology • Pre-filtration column for lysate clarification • Optimised alkaline lysis system • Supports high-copy and low-copy plasmids • Processes up to 400 mL bacterial culture • Supports plasmids up to approximately 40 kb • Approximately 30-minute vacuum workflow • Approximately 80–90-minute spin workflow • No phenol or chloroform extraction • No ethanol precipitation required

Sample Types: Bacterial Cultures • Recombinant E. coli Cultures • High-copy Plasmid Cultures • Low-copy Plasmid Cultures

Applications: PCR • Molecular Cloning • Automated DNA Sequencing • Manual DNA Sequencing • Transfection • Electroporation • Restriction Enzyme Digestion • In Vitro Translation • Preparation of Labelled Hybridisation Probes • Other Plasmid DNA-Based Research Applications

Research Use: Research Use Only (RUO)

Manufacturer: iNtRON Biotechnology

Technical Specifications

Note: The reported DNA recovery range is based on manufacturer experimental data and is not a guaranteed yield. Actual recovery depends on bacterial strain, culture medium, culture density, culture volume, plasmid copy number, plasmid size and insert characteristics.