PRODUCT INFORMATION
DNA-spin™ Plasmid DNA Purification Kit
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PRODUCT INFORMATION
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01 Description, applications & specifications
Description
DNA-spin™ Plasmid DNA Purification Kit from iNtRON Biotechnology provides a rapid and efficient method for isolating high-quality plasmid DNA from bacterial cultures using alkaline lysis and silica-membrane spin-column technology.
The kit is suitable for purification of both high-copy and low-copy plasmid DNA. Following bacterial cell resuspension, alkaline lysis and neutralisation, plasmid DNA selectively binds to the silica membrane under high-salt conditions. Contaminants are removed through washing steps, and purified plasmid DNA is then eluted in a small volume of low-salt elution buffer.
The included Lysis Viewer allows the pH changes occurring during cell lysis and neutralisation to be observed visually. This helps users confirm adequate mixing and reduces common handling errors associated with incomplete cell lysis or neutralisation.
The kit can provide up to 20 µg of highly purified plasmid DNA, while the specialised silica membrane has a maximum DNA-binding capacity of approximately 35 µg. It supports purification of both high-copy and low-copy plasmids, including plasmid DNA up to approximately 30 kb.
Purified plasmid DNA is free from major protein, RNA and chromosomal DNA contaminants and is ready for use in downstream molecular biology procedures without additional precipitation, concentration or desalting.
Key Features: Rapid plasmid DNA purification • Silica-membrane spin-column technology • Alkaline lysis buffer system • Suitable for high-copy and low-copy plasmids • Up to 20 µg plasmid DNA yield • Up to 35 µg column-binding capacity • Supports plasmids up to approximately 30 kb • Included Lysis Viewer • Approximately 30-minute workflow • No phenol-chloroform extraction • No alcohol precipitation required
Sample Types: Recombinant Bacterial Cultures • High-copy Plasmid Cultures • Low-copy Plasmid Cultures • Compatible E. coli Strains
Applications: Molecular Cloning • Ligation and Transformation • Conventional PCR • Real-time PCR • Restriction Enzyme Digestion • DNA Sequencing • Transfection • Microinjection • In Vitro Transcription • Other Plasmid DNA-Based Molecular Biology Applications
Research Use: Research Use Only (RUO)
Manufacturer: iNtRON Biotechnology
Technical Specifications
| Specification | Details |
|---|---|
| Product Name | DNA-spin™ Plasmid DNA Purification Kit |
| Catalogue Nos. | 17096 / 17097 / 17098 |
| Available Formats | 50 columns and 200 columns, including cap-column configuration |
| Target | Plasmid DNA |
| Sample Source | Bacterial cultures |
| Purification Technology | Silica-membrane spin column |
| Lysis Method | Alkaline lysis |
| Plasmid Types | High-copy and low-copy plasmids |
| Supported Plasmid Size | Up to approximately 30 kb |
| Typical Maximum Yield | Up to 20 µg plasmid DNA |
| Maximum Column-Binding Capacity | Approximately 35 µg DNA |
| Processing Time | Approximately 30 minutes |
| Process Indicator | Lysis Viewer for visual monitoring of lysis and neutralisation |
| Organic Extraction | Not required |
| Alcohol Precipitation | Not required |
| Downstream Applications | Cloning, ligation, transformation, PCR, real-time PCR, sequencing, restriction digestion, transfection and microinjection |
| Storage | Room temperature; store Resuspension Buffer at 2–8°C after adding RNase A |
| Classification | Research Use Only (RUO) |
| Manufacturer | iNtRON Biotechnology |
Note: Plasmid DNA yield may vary depending on bacterial strain, culture volume, plasmid copy number, plasmid size and culture conditions.
TECHNICAL LIBRARY
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