Eriksson (ER) medium including vitamins

Eriksson (ER) medium including vitamins by Duchefa Biochemie is supplied for plant tissue culture and laboratory research.

Description and application

Eriksson medium was developed for cell suspension cultures of Haplopappus gracilis. An increase in the growth of cell suspensions, especially with small inocula, was achieved by a 10% reduction of the concentration MS microelements, except for Fe and Zn. Equimolar replacement of ZnSO4.-7H2O by ZnNa2EDTA improved cell growth as well. MnEDTA and CoEDTA did not improve the growth of the cell culture. A reduction of Murashige and Skoog NH4NO3 concentration of 1650 to 1200 mg/l and an increase in phosphate to 2.5 mM also stimulated cell growth.

Composition and technical specifications

Micro Elements	mg/l	µM
CoCl2.6H2O	0.0025	0.01
CuSO4.5H2O	0.0025	0.01
FeNaEDTA	36.70	100.00
H3BO3	0.63	10.19
MnSO4.H2O	1.69	10.00
Na2MoO4.2H2O	0.025	0.10
ZnSO4.7H2O	9.15	31.80

Macro Elements	mg/l	mM
CaCl2	332.02	2.99
KH2PO4	340.00	2.50
KNO3	1900.00	18.79
MgSO4	180.54	1.50
NH4NO3	1200.00	14.99

Vitamins	mg/l	µM
Glycine	2.00	26.64
Nicotinic acid	0.50	4.06
Pyridoxine HCl	0.50	2.43
Thiamine HCl	0.50	1.48

Total concentration Micro and Macro elements including -vitamins: 4004.42 mg/l

Eriksson T., Physiol. Plant, 18, 976 (1965).

Hazard statements

• H315 Causes skin irritation
• H319 Causes serious eye irritation
• H335 May cause respiratory irritation

Precautionary statements

• P261 Avoid breathing dust/fume/gas/mist/vapours/spray
• P302 IF ON SKIN:
• P305 IF IN EYES:
• P351 Rinse continuously with water for several minutes
• P352 Wash with soap and water

Available pack options: 10 l, 50 l. Manufacturer reference: E0208.

Safety data sheet