PRODUCT INFORMATION
ExcelBand™ 3-Colour Pre-Stained Protein Ladder, Regular Range, 250 µL × 2
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PRODUCT INFORMATION
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01 Description, applications & specifications
Description
ExcelBand™ 3-Colour Pre-Stained Protein Ladder, Regular Range is a ready-to-use protein molecular-weight standard containing 13 pre-stained proteins for monitoring protein separation during SDS-PAGE and transfer during Western blotting.
The ladder provides three distinctive reference bands: red at 75 kDa, green at 40 kDa and enhanced blue at 20 kDa under Tris-Glycine conditions. It is premixed with loading buffer for direct gel loading without heating or boiling.
Key Features: Regular molecular-weight range • 13 pre-stained protein bands • Three-colour reference system • Red 75 kDa band • Green 40 kDa band • Enhanced blue 20 kDa band • Ready to use • No heating required • Compatible with Tris-Glycine, Bis-Tris MOPS and Bis-Tris MES systems • Suitable for SDS-PAGE and Western blotting
Applications: SDS-PAGE • Protein Molecular-Weight Estimation • Protein Separation Monitoring • Western Blotting • Transfer-Efficiency Verification • Protein Expression Analysis • General Protein Research
Compatibility: Suitable for Tris-Glycine and Bis-Tris gel systems using MOPS or MES running buffers. It can be visualised following transfer to PVDF, nylon or nitrocellulose membranes. Apparent molecular weights vary according to the gel and buffer system.
Packaging: Two vials containing 250 µL each, providing 500 µL total. Approximately 100 loads are available when using 5 µL per lane.
Manufacturer: SMOBIO Technology
Technical Specifications
| Specification | Details |
|---|---|
| SKU | PM5000 |
| Product Name | ExcelBand™ 3-Colour Pre-Stained Protein Ladder, Regular Range |
| Series | ExcelBand™ |
| Product Type | Pre-stained protein ladder |
| Product Size | 250 µL × 2 vials |
| Total Volume | 500 µL |
| Number of Bands | 13 |
| Tris-Glycine Range | Approximately 10–180 kDa |
| Bis-Tris MOPS Range | Approximately 9–170 kDa |
| Bis-Tris MES Range | Approximately 10–170 kDa |
| Band Colours | Blue, green and red |
| Reference Bands | 75 kDa red, 40 kDa green and 20 kDa enhanced blue under Tris-Glycine conditions |
| Protein Concentration | Approximately 0.1–0.4 mg/mL of each protein |
| Recommended Loading Volume | Approximately 5 µL per lane |
| Approximate Number of Loads | 100 loads at 5 µL per lane |
| Format | Ready to use; premixed with loading buffer |
| Heating Required | No |
| Buffer Composition | 20 mM Tris-phosphate, 2% SDS, 0.2 mM DTT, 3.6 M urea and 15% glycerol |
| Buffer pH | pH 7.5 at 25°C |
| Compatible Gel Systems | Tris-Glycine, Bis-Tris MOPS and Bis-Tris MES |
| Compatible Membranes | PVDF, nylon and nitrocellulose |
| Short-Term Storage | 4°C for up to 3 months |
| Long-Term Storage | −20°C |
| Manufacturer | SMOBIO Technology |
| Classification | Research Use Only (RUO) |
Note: The apparent molecular weight of pre-stained proteins varies with gel composition, running buffer and electrophoresis conditions. Use the migration pattern specified for the selected Tris-Glycine, MOPS or MES system.
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