PRODUCT INFORMATION
ExcelBand™ All Blue Broad Range Protein Marker, 9–240 kDa, 2 × 250 µL
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PRODUCT INFORMATION
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01 Description, applications & specifications
Description
ExcelBand™ All Blue Broad Range Protein Marker is a ready-to-use prestained standard containing 12 blue protein bands for estimating molecular weight and monitoring protein separation during SDS-PAGE.
The broad range supports analysis of proteins up to approximately 240 kDa. Enhanced 25 and 72 kDa bands provide quick references during electrophoresis and Western transfer.
Key Features: 12 prestained protein bands • All-blue band pattern • Approximate 9–240 kDa range • Enhanced 25 and 72 kDa reference bands • Ready to load • No boiling required • Broad molecular-weight coverage • Compatible with Tris-Glycine, Bis-Tris MOPS and Bis-Tris MES systems • Suitable for SDS-PAGE and Western blotting
Applications: SDS-PAGE • Broad-Range Protein Separation • Protein Molecular-Weight Estimation • Western Blotting • Transfer-Efficiency Monitoring • High-Molecular-Weight Protein Analysis • Membrane Transfer • Protein Research
Compatibility: Suitable for Tris-Glycine and Bis-Tris gel systems using MOPS or MES running buffer. Compatible with Western transfer onto PVDF, nylon and nitrocellulose membranes.
Packaging: 250 µL × 2 tubes, providing 500 µL total and approximately 100 applications when using 5 µL per lane.
Manufacturer: SMOBIO Technology
Technical Specifications
| Specification | Details |
|---|---|
| SKU | PM1700 |
| Product Name | ExcelBand™ All Blue Broad Range Protein Marker |
| Series | ExcelBand™ |
| Marker Type | Prestained broad-range protein molecular-weight marker |
| Number of Bands | 12 |
| Band Colour | All blue |
| Enhanced Reference Bands | 25 and 72 kDa in Tris-Glycine SDS-PAGE |
| Tris-Glycine Range | Approximately 10–240 kDa |
| Bis-Tris MOPS Range | Approximately 9–235 kDa |
| Bis-Tris MES Range | Approximately 9–235 kDa |
| Protein Concentration | Approximately 0.1–0.5 mg/mL of each protein |
| Chromophore | Blue chromophore covalently coupled to the marker proteins |
| Format | Ready to use; premixed with loading buffer |
| Heating Required | No boiling or heating required |
| Recommended Loading Volume | Approximately 5 µL per lane |
| Pack Size | 250 µL × 2 tubes; 500 µL total |
| Approximate Number of Loads | 100 loads at 5 µL per lane |
| Buffer Composition | 20 mM Tris-phosphate, 2% SDS, 0.2 mM DTT, 3.6 M urea and 15% glycerol |
| Buffer pH | pH 7.5 at 25°C |
| Electrophoresis Compatibility | Tris-Glycine, Bis-Tris MOPS and Bis-Tris MES SDS-PAGE |
| Western Blot Compatibility | PVDF, nylon and nitrocellulose membranes |
| Short-Term Storage | 4°C for up to 3 months |
| Long-Term Storage | −20°C |
| Manufacturer | SMOBIO Technology |
| Classification | Research Use Only (RUO) |
Note: Prestaining and differences in gel chemistry can affect apparent protein migration. Use the molecular-weight values specified for the selected buffer system and treat the marker as an approximate size reference.
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