PRODUCT INFORMATION
ExcelTaq™ 2X PCR Master Mix with MgSO₄, 100 RXN
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PRODUCT INFORMATION
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01 Description, applications & specifications
Description
ExcelTaq™ 2X PCR Master Mix with MgSO₄ is a ready-to-use formulation containing recombinant Taq DNA polymerase, reaction buffer, MgSO₄, dNTPs and enzyme stabiliser. Only primers, template DNA and water need to be added.
The mix supports reliable amplification of DNA fragments up to 8 kb. A separate 6X blue DNA loading dye containing two tracking dyes is supplied for post-PCR agarose-gel electrophoresis.
Key Features: Ready-to-use 2X formulation • MgSO₄-based PCR buffer • Recombinant Taq DNA polymerase • Includes dNTPs and stabiliser • High amplification yield • High reproducibility • Reduced pipetting errors • Amplification up to 8 kb • Produces 3′-dA overhangs • Separate blue loading dye included
Applications: Routine PCR • Colony PCR • High-Throughput PCR • DNA Amplification up to 8 kb • TA Cloning • DNA Labelling • Agarose-Gel Electrophoresis • Molecular Biology Research
Compatibility: Suitable for standard thermal cyclers and PCR applications compatible with a MgSO₄-containing buffer. The separately supplied loading dye may be added to completed PCR products before agarose-gel electrophoresis.
Packaging: ExcelTaq™ 2X PCR Master Mix with MgSO₄, 1.25 mL × 2 tubes • 6X DNA Loading Dye (Blue), 1 mL • Approximately 100 reactions.
Manufacturer: SMOBIO Technology
Technical Specifications
| Specification | Details |
|---|---|
| SKU | TP1120 |
| Product Name | ExcelTaq™ 2X PCR Master Mix (MgSO₄) |
| Product Format | Ready-to-use PCR master mix |
| Concentration | 2X |
| Polymerase | Recombinant Taq DNA polymerase |
| Master Mix Components | Taq DNA polymerase, reaction buffer, MgSO₄, dNTPs and enzyme stabiliser |
| Magnesium Salt | Magnesium sulphate; MgSO₄ |
| 5′→3′ DNA Polymerase Activity | Yes |
| 3′→5′ Exonuclease Activity | Not detectable; no proofreading activity |
| PCR Product Ends | 3′-dA overhangs |
| Maximum Recommended Target Length | Up to 8 kb |
| Master Mix Volume | 1.25 mL × 2 tubes; 2.5 mL total |
| Number of Reactions | Approximately 100 reactions |
| Loading Dye | 6X DNA Loading Dye (Blue), supplied separately |
| Loading Dye Volume | 1 mL |
| Tracking Dyes | Xylene cyanol FF and bromophenol blue |
| Dye Incorporated into Master Mix | No; add the supplied loading dye after amplification when required |
| Typical Reaction Volume | 50 µL |
| Master Mix per 50 µL Reaction | 25 µL |
| Recommended Template Amount | 1–150 ng per 50 µL reaction |
| Recommended Primer Concentration | 0.1–0.5 µM each |
| Components Added by User | Forward primer, reverse primer, template DNA and nuclease-free water |
| Initial Denaturation | 94°C for 2 minutes |
| Typical Cycling | 25–40 cycles |
| Denaturation | 94°C for 30 seconds |
| Annealing | 50–68°C for 30 seconds; optimise for the selected primers |
| Extension | 72°C for approximately 30 seconds per kb |
| Final Extension | 72°C for 1 minute |
| Storage at 4°C | Up to 6 months |
| Storage at −20°C | Up to 24 months |
| Manufacturer | SMOBIO Technology |
| Classification | Research Use Only (RUO) |
Note: TP1120 uses MgSO₄ rather than the MgCl₂ used in TP1100. Magnesium salt and concentration can influence primer annealing, enzyme activity and amplification specificity, so conditions may require optimisation when transferring an existing assay.
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