PRODUCT INFORMATION
ExcelTaq™ Klen-Taq DNA Polymerase, 5 U/µL, 500 U
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PRODUCT INFORMATION
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01 Description, applications & specifications
Description
ExcelTaq™ Klen-Taq DNA Polymerase is an enzyme blend containing KlenTaq-1 DNA polymerase and a small amount of proofreading polymerase for improved PCR fidelity, yield and processivity.
The enzyme provides approximately fourfold higher fidelity than Taq and is recommended for genomic targets of 0.5–5 kb and less-complex templates up to 10 kb. It produces both 3′-dA-overhang and blunt-ended amplicons.
Key Features: KlenTaq-1 and proofreading-polymerase blend • Approximately fourfold higher fidelity than Taq • Robust PCR performance • Tolerant of magnesium variation • Targets up to 5 kb from genomic DNA • Targets up to 10 kb from less-complex templates • Mixed 3′-dA and blunt-ended products • Suitable for TA cloning
Applications: Routine PCR • Enhanced-Fidelity PCR • Genomic DNA Amplification • Long-Range PCR • TA Cloning • Gene Cloning • PCR Product Analysis • DNA Sequencing Template Preparation
Compatibility: Suitable for PCR applications requiring greater fidelity than conventional Taq while maintaining strong yield and reaction robustness. Amplicons are directly compatible with TA-cloning workflows.
Packaging: 100 µL enzyme at 5 U/µL, providing 500 units and approximately 200 standard reactions at 2.5 units per reaction. Supplied with 1.2 mL of 10X Klen Buffer.
Manufacturer: SMOBIO Technology
Technical Specifications
| Specification | Details |
|---|---|
| SKU | TK1000 |
| Product Name | ExcelTaq™ Klen-Taq DNA Polymerase |
| Product Type | Enhanced-fidelity DNA polymerase blend |
| Enzyme Composition | KlenTaq-1 DNA polymerase with a trace amount of proofreading DNA polymerase |
| KlenTaq-1 Description | 5′-exonuclease-minus, N-terminal deletion of Taq DNA polymerase |
| Enzyme Concentration | 5 U/µL |
| Total Enzyme Quantity | 500 U |
| Enzyme Volume | 100 µL |
| Chemical Modification | None |
| 5′→3′ DNA Polymerase Activity | Yes |
| 3′→5′ Exonuclease Activity | Present through the proofreading-polymerase component |
| Relative Fidelity | Approximately four times higher than Taq DNA polymerase |
| PCR Product Ends | Mixture of 3′-dA overhangs and blunt ends |
| TA-Cloning Compatibility | Yes; amplicons are directly suitable |
| Recommended Genomic DNA Target | Approximately 0.5–5 kb |
| Recommended Less-Complex Template Target | Up to 10 kb |
| Maximum Demonstrated Target | Up to 12 kb from λDNA |
| Template Sensitivity | Amplification demonstrated from as little as 1 pg DNA |
| Denaturation Tolerance | Up to 98°C |
| Typical Reaction Volume | 50 µL |
| Recommended Enzyme per Reaction | 2.5 U |
| Recommended Template Amount | 1–150 ng per 50 µL reaction |
| Recommended Primer Concentration | 0.1–0.5 µM each |
| Recommended dNTP Concentration | 0.2 mM each |
| Recommended Extension Temperature | 68°C |
| Recommended Extension Time | 30 seconds per kb |
| 10X Klen Buffer | 1.2 mL |
| 10X Buffer Composition | 400 mM Tricine-KOH, 150 mM potassium acetate, 35 mM magnesium acetate and 750 µg/mL BSA |
| Approximate Enzyme Reaction Capacity | 200 reactions at 2.5 U per reaction |
| Approximate Buffer Capacity | 240 standard 50 µL reactions |
| Enzyme Storage Buffer | 40 mM Tris-HCl, 50 mM KCl, 25 mM ammonium sulphate, 0.1 mM EDTA, 5 mM 2-mercaptoethanol, stabiliser and 50% glycerol |
| Unit Definition | Amount incorporating 10 nmol dNTP into acid-insoluble material in 30 minutes at 74°C |
| Storage Temperature | −20°C |
| Stated Stability | 24 months at −20°C |
| Manufacturer | SMOBIO Technology |
| Classification | Research Use Only (RUO) |
Note: The manufacturer recommends targets up to 5 kb from genomic DNA and up to 10 kb from less-complex templates. The 12 kb result was demonstrated using λDNA and should not be treated as a universal amplification limit.
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