PRODUCT INFORMATION
ExcelTaq™ Taq DNA Polymerase, 5 U/µL, 500 U
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PRODUCT INFORMATION
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01 Description, applications & specifications
Description
ExcelTaq™ Taq DNA Polymerase is a recombinant thermostable enzyme designed for routine and specialised PCR applications requiring reliable DNA amplification and high product yield.
The enzyme provides 5′→3′ polymerase and exonuclease activities without detectable proofreading activity. It generates PCR products with 3′-dA overhangs suitable for direct TA cloning.
Key Features: Recombinant Taq DNA polymerase • 5′→3′ polymerase activity • No detectable proofreading activity • 3′-dA-overhang products • Amplification up to 8 kb • High thermostability • Sensitivity down to 1 fg DNA • Suitable for TA cloning • Approximately 400 standard reactions
Applications: Routine PCR • DNA Amplification • Genomic DNA PCR • cDNA Amplification • Colony PCR • PCR Product Labelling • TA Cloning • DNA Sequencing Template Preparation
Compatibility: Suitable for routine PCR applications where high yield and 3′-dA-overhang products are required. It is not recommended when high-fidelity proofreading is essential.
Packaging: 100 µL enzyme at 5 U/µL, providing 500 units and approximately 400 standard reactions at 1.25 units per reaction. Supplied with two 1 mL bottles of 10X Taq Buffer.
Manufacturer: SMOBIO Technology
Technical Specifications
| Specification | Details |
|---|---|
| SKU | TP1000 |
| Product Name | ExcelTaq™ Taq DNA Polymerase |
| Product Type | Standard Taq DNA polymerase |
| Enzyme Source | Recombinant thermostable Taq DNA polymerase expressed in E. coli |
| Enzyme Concentration | 5 U/µL |
| Total Enzyme Quantity | 500 U |
| Enzyme Volume | 100 µL |
| 5′→3′ DNA Polymerase Activity | Yes |
| 5′→3′ Exonuclease Activity | Yes |
| 3′→5′ Exonuclease Activity | Not detectable; no proofreading activity |
| PCR Product Ends | 3′-dA overhangs |
| TA-Cloning Compatibility | Yes |
| Recommended Maximum Target Length | Up to 8 kb |
| Maximum Demonstrated Target | Up to 15 kb from λDNA |
| Template Sensitivity | Amplification demonstrated from as little as 1 fg DNA |
| Thermostability | Half-life greater than 40 minutes at 95°C |
| Typical Reaction Volume | 50 µL |
| Recommended Enzyme per Reaction | 1.25 U |
| Recommended Template Amount | 1–150 ng per 50 µL reaction |
| Recommended Primer Concentration | 0.1–0.5 µM each |
| Recommended dNTP Concentration | 0.2 mM each |
| Recommended Extension Temperature | 72°C |
| Recommended Extension Time | 30 seconds per kb |
| 10X Taq Buffer | 1 mL × 2 bottles |
| 10X Buffer Composition | 200 mM Tris-HCl, 100 mM KCl, 100 mM ammonium sulphate, 20 mM MgCl₂ and 1% Triton X-100 |
| Final MgCl₂ Concentration at 1X | 2 mM |
| Approximate Reaction Capacity | 400 standard 50 µL reactions |
| Enzyme Storage Buffer | 20 mM Tris-HCl, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabiliser and 50% glycerol |
| Unit Definition | Amount incorporating 10 nmol dNTP into acid-insoluble material in 30 minutes at 74°C |
| Storage Temperature | −20°C |
| Stated Stability | 24 months at −20°C |
| Manufacturer | SMOBIO Technology |
| Classification | Research Use Only (RUO) |
Note: The recommended amplification range is up to 8 kb. The 15 kb result was demonstrated using λDNA and should not be treated as a universal amplification limit. Use a proofreading polymerase when sequence accuracy is critical.
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