Ez C-cell E. coli DH5α

Description

Ez C-cell E. coli DH5α is a high-performance Competent Cell produced using a chemical modification method, offering the optimal solution for cloning and library construction. With a transformation efficiency of 10⁸ CFU/µg of DNA, this product is designed to enable researchers to perform DNA cloning more easily and rapidly.

To maximize user convenience, Ez C-cell is provided in individual C-cell units, allowing researchers to use only the required amount and efficiently store any leftovers. Additionally, it comes with SOC broth for recovery culture and plating beads that facilitate easy spreading on agar plates, minimizing hassle during the experimental process. These features significantly enhance experimental consistency and improve the overall user experience.

One of the standout aspects of Ez C-cell is its Superfast Protocol which reduces the traditional cloning process, typically takng 1 hour and 40 minutes, to just 5 minutes. This time-saving capability allows researchers to maximize their productivity and conduct more experiments efficently.

Ez C-cell E. coli DH5α supports users in achieving quick, reliable, and consistent cloning results, thereby increasing the success rate of experiments and maximizing research efficiency. This product sets a new standard for cloning workflows, promising better outcomes for researchers.

Characteristics

• High Transformation Efficiency

• Ease of Use 

• Superfast Protocol 

• Convenient Plating Function

• Time Savings

• Cost Efficiency

Convenient Product Features

• Competent Cells

• SOC Broth

• Plating Beads

• Control Plasmid

Kit Contents

Storage

• Should be stored at or below -80°C until the expiration date indicated

• Avoid freeze-thaw cycles and do not reuse

Analysis Workflow

Technical Data

1. Transformation Efficiency of Ez C-cell E. coli DH5α

• An evaluation was conducted on the transformation efficiency of Ez C-cell E. coli DH5α using cloning vectors, protein expression vectors, and a long-length adenoviral-based vector.

• The representative cloning vector, pUC19, demonstrated a transformation efficiency of approximately 1.9 x 10⁸ CFU/µg, while the 33.4 kb long pAdEasy-1 vector showed a transformation efficiency of about 2.9 x 10⁶ CFU/µg.

• These results indicate performance that is equal to or exceeds that of similar products from other sources. (The above test data is provided as our experimental result, and variations may occur depending on the quality of the DNA.)

2. Effect of Spreading Beads

• Ez C-cell products provide Plating beads to assist with spreading after transformation.

• For a single use, 5 to 6 beads are added, and the plate is gently shaken to perform the spreading process.

• Using Plating beads helps to distribute colonies more evenly across the plate, allowing for approximately 1.4 times more colonies to be observed compared to traditional methods using a spreader.

• The product includes enough beads for 20 spreading applications.

Available pack options: 20T(50 μl x 20 ea). Manufacturer reference: 15065.