PRODUCT INFORMATION
FluoroBand™ 100 bp + 3 kb Fluorescent DNA Ladder, 500 µL
UK/EU ordering: add this item to cart, review your order, then continue securely to checkout.
PRODUCT INFORMATION
Clear technical information,
without the clutter.
01 Description, applications & specifications
Description
FluoroBand™ 100 bp + 3 kb Fluorescent DNA Ladder is a ready-to-use DNA molecular-size standard containing 12 fragments from 100 bp to 3 kb. It is premixed with fluorescent DNA-binding dye and loading dye for direct gel loading and visualisation.
The 500 bp and 1.5 kb fragments have enhanced intensity for quick orientation. Bands can be visualised using 470 nm blue light or UV illumination, while two tracking dyes enable real-time monitoring during electrophoresis.
Key Features: 100 bp–3 kb range • 12 fluorescent DNA fragments • Enhanced 500 bp and 1.5 kb reference bands • Ready to use • Premixed with fluorescent DNA-binding dye • Premixed with loading dye • Blue-light or UV visualisation • Two electrophoresis tracking dyes • Recommended loading volume of 5 µL per well
Applications: Agarose Gel Electrophoresis • Fluorescent DNA Visualisation • DNA Fragment Sizing • PCR Product Analysis • Restriction Digest Analysis • Molecular Cloning • Genotyping • General Molecular Biology Research
Compatibility: Suitable for agarose gel electrophoresis and visualisation using 470 nm blue-light or UV transilluminators. Real-time observation is possible when a compatible light source is fitted to the electrophoresis system.
Packaging: One light-protective vial containing 500 µL of ready-to-use fluorescent DNA ladder.
Manufacturer: SMOBIO Technology
Technical Specifications
| Specification | Details |
|---|---|
| SKU | DM2360 |
| Product Name | FluoroBand™ 100 bp + 3 kb Fluorescent DNA Ladder |
| Series | FluoroBand™ |
| Product Type | Ready-to-use fluorescent DNA ladder |
| Product Size | 500 µL |
| Size Range | 100–3,000 bp |
| Number of Bands | 12 |
| DNA Fragment Sizes | 3,000, 1,500, 1,000, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp |
| Enhanced Bands | 500 bp and 1,500 bp |
| Total DNA Concentration | 56 µg per 500 µL |
| Recommended Loading Volume | 5 µL per well |
| Approximate Number of Loads | 100 loads at 5 µL per well |
| Format | Ready to use; premixed with fluorescent DNA-binding dye and loading dye |
| Visualisation | 470 nm blue light or UV illumination |
| Tracking Dyes | Orange G and xylene cyanol FF |
| Orange G Migration | Approximately equivalent to 50 bp double-stranded DNA |
| Xylene Cyanol FF Migration | Approximately equivalent to 4,000 bp double-stranded DNA |
| DNA Source | Phenol-extracted PCR products and double-stranded DNA digested with specific restriction enzymes |
| Buffer | 10 mM Tris-HCl, pH 8.0, and 10 mM EDTA |
| Light Protection | Required |
| Room-Temperature Stability | 6 months when protected from light |
| Stability at 4°C | 12 months when protected from light |
| Stability at −20°C | 24 months when protected from light |
| Manufacturer | SMOBIO Technology |
| Classification | Research Use Only (RUO) |
Note: Protect the ladder from prolonged light exposure to preserve fluorescence. DNA migration and apparent fragment size may vary with agarose concentration, electrophoresis buffer, voltage and running time.
TECHNICAL LIBRARY
Product documents
Open the available technical and product literature for this item.
Need help selecting the correct product or pack size?Our team can review your application, compatibility and supply requirements.