PRODUCT INFORMATION
i-Pfu™ DNA Polymerase with Buffer, dNTPs
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PRODUCT INFORMATION
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01 Description, applications & specifications
Description
i-Pfu™ DNA Polymerase with Buffer, dNTPs is a high-fidelity, thermostable DNA polymerase from iNtRON Biotechnology designed for PCR applications requiring accurate DNA synthesis. The enzyme is derived from Pyrococcus furiosus DNA polymerase and catalyses DNA synthesis in the 5′→3′ direction.
i-Pfu™ DNA Polymerase possesses 3′→5′ exonuclease proofreading activity, allowing incorrectly incorporated nucleotides to be removed during DNA synthesis. This produces PCR products with greater sequence accuracy than conventional Taq DNA polymerase and makes the enzyme particularly suitable for cloning, expression studies, mutagenesis and amplification of templates intended for DNA sequencing.
The polymerase produces blunt-ended amplification products and is recommended for high-fidelity amplification of genomic DNA and cDNA targets up to 10 kb. It can be used with a variety of DNA templates, including cloned fragments, plasmid DNA, phage DNA, cDNA and mammalian genomic DNA.
The product is supplied at a concentration of 2.5 U/µL together with 10X PCR Buffer containing Mg²⁺, 10X Mg²⁺-free PCR Buffer, dNTP mixture and a separate MgCl₂ solution. These components provide flexibility for optimization of magnesium concentration and other PCR conditions.
Key Features: High-fidelity DNA amplification • 3′→5′ exonuclease proofreading activity • Low nucleotide-incorporation error rate • Blunt-ended PCR products • Amplification of targets up to 10 kb • Suitable for genomic DNA and cDNA • Flexible magnesium optimization • Supplied with PCR buffers, dNTPs and MgCl₂ • Suitable for applications requiring accurate DNA synthesis
Applications: High-Fidelity PCR • Genomic DNA Amplification • cDNA Amplification • Gene Cloning • Blunt-End Cloning • DNA Sequencing Template Preparation • Gene Expression Studies • Site-Directed Mutagenesis • Primer Extension • General Molecular Biology Research
Included Components: i-Pfu™ DNA Polymerase (2.5 U/µL) • 10X PCR Buffer with 15 mM Mg²⁺ • 10X Mg²⁺-free PCR Buffer • 10 mM dNTP Mixture (2.5 mM each) • 25 mM MgCl₂ Solution
Storage: Store at −20°C. Under the recommended storage conditions, the manufacturer states that the product is stable for at least one year.
Research Use: Research Use Only (RUO). Not intended for diagnostic or clinical use.
Manufacturer: iNtRON Biotechnology
Technical Specifications
| Specification | Details |
|---|---|
| Catalogue No. | 25181 |
| Pack Size | 250 units |
| Enzyme | i-Pfu™ DNA Polymerase |
| Enzyme Source | Recombinant enzyme based on Pyrococcus furiosus DNA polymerase |
| Enzyme Concentration | 2.5 U/µL |
| Polymerase Activity | 5′→3′ DNA synthesis |
| 3′→5′ Exonuclease Activity | Yes; proofreading activity |
| 5′→3′ Exonuclease Activity | Not detectable |
| PCR Product Ends | Blunt ends |
| Recommended Target Length | Up to 10 kb |
| Recommended Reaction Volume | 50 µL |
| Recommended Enzyme per Reaction | 1.25–2.5 units for a typical 50 µL reaction |
| 10X PCR Buffer with Mg²⁺ | 1 mL; contains 15 mM Mg²⁺ at 10X concentration |
| 10X Mg²⁺-Free PCR Buffer | 1 mL |
| dNTP Mixture | 10 mM formulation; 2.5 mM of each dNTP, 500 µL |
| MgCl₂ Solution | 25 mM, 1 mL |
| Recommended Extension Temperature | 65–72°C |
| Storage Temperature | −20°C |
| Classification | Research Use Only (RUO) |
TECHNICAL LIBRARY
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