i-StarTaq™ DNA Polymerase with Buffer, dNTPs

Description

i-StarTaq™ DNA Polymerase with Buffer, dNTPs is a chemically mediated Hot-Start Taq DNA Polymerase from iNtRON Biotechnology. The enzyme is designed to remain inactive at lower temperatures and become activated during the high-temperature stage of PCR, helping minimize premature primer extension, primer-dimer formation and nonspecific amplification.

Its Hot-Start performance supports PCR amplification with improved specificity, sensitivity and reproducibility, particularly when working with difficult primer-template combinations. The enzyme is suitable for amplification of genomic DNA and cDNA targets up to 5 kb, as well as templates containing GC-rich regions or secondary structures.

The enzyme is supplied at a concentration of 5 U/µL together with 10X PCR Buffer containing Mg²⁺, 10X Mg²⁺-free PCR Buffer, dNTP mixture and a separate MgCl₂ solution. The two buffer options and separate magnesium solution allow reaction conditions to be optimized for different templates and primer sets.

Key Features: Chemically mediated Hot-Start Taq DNA Polymerase • Reduced nonspecific amplification • Reduced primer-dimer formation • High specificity and sensitivity • Reproducible PCR results • Suitable for difficult and GC-rich templates • Amplification of targets up to 5 kb • Flexible magnesium optimization • Supplied with PCR buffers, dNTPs and MgCl₂

Applications: Hot-Start PCR • Genomic DNA PCR • cDNA Amplification • RT-PCR • Pathogen Research • GC-Rich Template PCR • Difficult-Template PCR • T/A Cloning • Blunt-End Cloning • LOH and MSI Research • General Molecular Biology

Included Components: i-StarTaq™ DNA Polymerase (5 U/µL) • 10X PCR Buffer with 20 mM Mg²⁺ • 10X Mg²⁺-free PCR Buffer • 10 mM dNTP Mixture (2.5 mM each) • 25 mM MgCl₂ Solution

Storage: Store at −20°C.

Research Use: Research Use Only (RUO). Not intended for diagnostic or clinical use.

Manufacturer: iNtRON Biotechnology

Technical Specifications