i-Taq™ DNA Polymerase with Buffer and dNTPs

Description

i-Taq™ DNA Polymerase with Buffer and dNTPs from iNtRON Biotechnology is a highly purified, thermostable DNA polymerase for routine PCR amplification of DNA targets up to approximately 5 kb.

i-Taq™ is a recombinant 94 kDa Taq DNA polymerase derived from the polymerase gene of Thermus aquaticus strain YT-1 and expressed in E. coli. The purification process is designed to remove residual host-derived proteins and DNA that could otherwise contribute to contamination or non-specific amplification.

The enzyme is supplied at a concentration of 5 U/µL together with a 10X PCR Buffer containing MgCl₂, a separate 10X MgCl₂-free PCR Buffer, a dNTP mixture and 25 mM MgCl₂. This flexible component format allows users to perform standard PCR using the MgCl₂-containing buffer or optimise the magnesium concentration for demanding templates and primer sets using the MgCl₂-free buffer and separately supplied MgCl₂ solution.

i-Taq™ DNA Polymerase has optimal DNA synthesis activity at approximately 72°C. It is suitable for amplification from genomic DNA and cDNA templates, routine PCR, RT-PCR-derived cDNA amplification, direct sequencing-related PCR, T/A cloning and PCR assays used for loss-of-heterozygosity or microsatellite-instability research.

As a conventional Taq DNA polymerase, i-Taq™ does not possess 3′→5′ proofreading exonuclease activity and typically produces PCR products with 3′-adenine overhangs. This makes the amplified products suitable for T/A cloning. For applications requiring substantially higher sequence fidelity, a proofreading polymerase such as i-pfu™ or an enzyme blend such as i-MAX™ II should be considered.

Key Features: Standard recombinant Taq DNA polymerase • 94 kDa thermostable enzyme • 5 U/µL concentration • Amplifies DNA targets up to approximately 5 kb • Optimal extension at approximately 72°C • Supplied with dNTP mixture • Includes MgCl₂-containing and MgCl₂-free PCR buffers • Separate MgCl₂ solution for optimisation • Suitable for genomic DNA and cDNA templates • Produces PCR products suitable for T/A cloning • Flexible individual-component format

Template Types: Genomic DNA • cDNA • Plasmid DNA • Purified DNA • Cloned DNA Templates • Other Compatible DNA Templates

Applications: Routine PCR • Genomic DNA PCR • cDNA Amplification • RT-PCR-Related Amplification • Colony PCR • Direct Sequencing-Related PCR • T/A Cloning • Loss-of-Heterozygosity Research • Microsatellite-Instability Research • Other Standard PCR Applications

Research Use: Research Use Only (RUO)

Manufacturer: iNtRON Biotechnology

Technical Specifications

Kit Contents

Note: Maximum amplicon length and PCR performance depend on template quality, target GC content, primer design, magnesium concentration, cycle conditions and reaction optimisation. For high-fidelity cloning or sequence-critical applications, use an appropriate proofreading polymerase.