KAO & Michayluk medium

KAO & Michayluk medium by Duchefa Biochemie is supplied for plant tissue culture and laboratory research.

Description and application

The medium defined by Kao and Michayluk was designed to grow cells and protoplasts of Vicia hajastana at a very low population -density in liquid media. The inability of the plant cells to grow at a very low population density may be caused by excessive diffusion of metabolic intermediates into the medium, resulting in their dilution in the cell to a level below that required for survival. Vicia cells were able to grow at an initial population density of 1-2 cells/ml when the mineral salt medium was enriched with organic acids, sugars, sugar alcohols, growth regulators, amino acids and other organic compounds. The percentage of cell division could be increased by raising the -concentration of CaCl2 from 1 mM, as in Gamborg B5, to 5 mM. Calcium may play an important role in the proces of cell division because of its ability to preserve the structural and functional integrity of plant cell membranes. The medium defined by Kao and Michayluk was designed to grow cells and protoplasts of Vicia hajastana at a very low population -density in liquid media. The inability of the plant cells to grow at a very low population density may be caused by excessive diffusion of metabolic intermediates into the medium, resulting in their dilution in the cell to a level below that required for survival. Vicia cells were able to grow at an initial population density of 1-2 cells/ml when the mineral salt medium was enriched with organic acids, sugars, sugar alcohols, growth regulators, amino acids and other organic compounds. The percentage of cell division could be increased by raising the -concentration of CaCl2 from 1 mM, as in Gamborg B5, to 5 mM. Calcium may play an important role in the proces of cell division because of its ability to preserve the structural and functional integrity of plant cell membranes.

Composition and technical specifications

Micro Elements	mg/l	µM
CoCl2.6H2O	0.025	0.11
CuSO4.5H2O	0.025	0.10
FeNaEDTA	36.70	100.00
H3BO3	3.00	48.52
KI	0.75	4.52
MnSO4.H2O	10.00	59.17
Na2MoO4.2H2O	0.25	1.03
ZnSO4.7H2O	2.00	6.96

Macro Elements	mg/l	mM
CaCl2	453.00	4.08
KCl	300.00	4.02
KH2PO4	170.00	1.25
KNO3	1900.00	18.79
MgSO4	146.84	1.22
NH4NO3	600.00	7.50

Kao K.N., O.L. Gamborg et al., The effects of sugars and inorganic salts on cell regeneration and sustained division in plant proto-plasts. Colloques internationaux C.N.R.S., 212, Protoplastes et fusion de cellules somatiques végétales.Kao K.N. and Michayluk M.R., Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta (Berl.), 126, 105 (1975).

Hazard statements

• H315 Causes skin irritation
• H319 Causes serious eye irritation
• H335 May cause respiratory irritation

Precautionary statements

• P261 Avoid breathing dust/fume/gas/mist/vapours/spray
• P302 IF ON SKIN:
• P305 IF IN EYES:
• P351 Rinse continuously with water for several minutes
• P352 Wash with soap and water

Available pack options: 5 l, 10 l. Manufacturer reference: K0214.

Safety data sheet