PRODUCT INFORMATION
LiliF® Salmonella Real-time PCR Kit (ver.2.0)
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PRODUCT INFORMATION
Clear technical information,
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01 Description, applications & specifications
Description
Background Information
Salmonella spp. Food microbiological test kit with microbiological test method
Products applied with the Food Standards Microbial Test Method of Salmonella spp.
High real-time PCR equipment versatility of master mix type
Application of UNG system to prevent false positive detection
Implementation of PCR reaction validation through IPC system.
LiliF® Salmonella Real-time PCR Kit (ver2.0) is a product that implements the microbial test method of the Food Standards Code that can specifically detect the invA gene of Salmonella spp. (Revised in April 2022, Food Standards ▶ Article 8. General test method ▶ 4. Microbial test method ▶ 4.26 Real-time PCR method of molecular biological test method for food poisoning bacteria). It is a versatile master mix type product that can detect target genes up to 10 copy/uL and can use various real-time PCR equipment. LiliF® Salmonella Real-time PCR Kit (ver2.0) is a product that maximizes performance improvement and PCR efficiency by optimizing the food industry’s invA-specific primer/probe with its RealMOD™Probe M2 2X qPCR mix (with UDG). Uracil DNA Glycosylase (UDG) was added to verify the reliability and validity of the results, and the UNG system was applied to prevent false-positive results from cross-over contamination by PCR amplicons. Internal positive control (IPC) DNA that does not affect target detection and IPC-specific primers/probes are added to the product to confirm IPC detection regardless of target amplification, so you can check the effectiveness of the PCR reaction.
Applications
• For research Use Only, Not for use in diagnostic procedures.• The Kit is developed, designed, and sold for research purpose only.• In-process monitoring for the presence of Salmonella spp.Intended Use
• This product is a kit for the detection of harmful microorganisms in various foods and livestock products.
• It is a reagent for qualitative analysis by real-time PCR test for the presence of Salmonella typhimurium or Salmonella enteritidis in microorganism culture.
Kit Contents
| No. | Contents | 50 Tests/Kit |
|---|---|---|
| 1 | 2X PCR Mix | 560 μl x 1 tube |
| 2 | Salmonella Detection Sol. | 280 μl x 1 tube |
| 3 | Positive Control | 25 μl x 3 tubes |
| 4 | DNase/RNase Free Water | 1 ml x 1 tube |
Technical Information
Performance
● Target nucleic acid detection result invA gene: 10 copy/uL detection limit confirmed
Available pack options: 50 tests. Manufacturer reference: IP24737.
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