LiliF® VTEC Real-time PCR Kit (ver.2.0)

Description

Background Information

Products applied with the Food Standards Microbiological Test Method of Vero toxin producing E. coli (VTEC, Vero toxin producing E. coli)High real-time PCR equipment versatility of master mix typeApplication of UNG system to prevent false positive detectionImplementation of PCR reaction validation through IPC system​
LiliF® VTEC Real-time PCR Kit (ver2.0) is a product that implements the Korea Food Codex's intestinal hemorrhagic E. coli microorganism test method that can specifically detect Vero toxin gene stx1/stx2 (revised in April 2022, food Standard ▶ Article 8. General Test Method ▶ 4. Microbial Test Method ▶ 4.16 Real-time PCR Method of Enterohaemorrhagic E. coli). It is a versatile master mix type product that can detect up to 10 copy/uL and 1 copy/uL of each target gene and can use a variety of real-time PCR equipment. LiliF® VTEC Real-time PCR Kit (ver2.0) is a product that maximizes performance improvement and PCR efficiency by optimizing the food industry's stx1/2 specific primer/probe with its RealMOD™Probe M2 2X qPCR mix (with UDG). Uracil DNA Glycosylase (UDG) was added to verify the reliability and validity of the test results, and the UNG system was applied to prevent false-positive results from cross-over contamination by PCR amplicons. Internal positive control (IPC) DNA that does not affect target detection and IPC-specific primers/probes are added to the product to confirm IPC detection regardless of target amplification, so you can check the effectiveness of the PCR reaction.

Applications

•​  For research Use Only, Not for use in diagnostic procedures.•​  The Kit is developed, designed, and sold for research purpose only.•​  In-process monitoring for the presence of Vero toxin producing E.coli.

Kit Contents

No.	Contents	50 Tests/Kit
1	2X PCR Mix	560 μl x 1 tube
2	VTEC Detection Sol.	280 μl x 1 tube
3	Positive Control	25 μl x 3 tubes
4	DNase/RNase Free Water	1 ml x 1 tube

Technical Information

Performance

● Result of detection of target nucleic acid VT1 : 10 copy/uL, VT2 : 1 copy/uL Confirmation of detection limit 

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Available pack options: 50 tests. Manufacturer reference: IP24739.