PRODUCT INFORMATION
Q-PAGE™ TGN Precast Gel (Mini, 12 Wells, 4–15%), 10 Gels
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PRODUCT INFORMATION
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01 Description, applications & specifications
Description
Q-PAGE™ TGN Precast Gel – Mini, 12 Wells, 4–15%, 10 Gels is a ready-to-use gradient polyacrylamide gel system from SMOBIO Technology for protein separation by SDS-PAGE using the conventional Tris-Glycine buffer system.
The proprietary TGN, or Tris-Glycine Novel, formulation provides rapid electrophoresis, clear protein-band separation and extended storage stability compared with conventional manually prepared Laemmli Tris-HCl gels. Protein migration patterns remain comparable to traditional Tris-Glycine gels, supporting convenient integration into established SDS-PAGE workflows.
The 4–15% polyacrylamide gradient is suitable for broad-range separation of protein mixtures containing different molecular weights. The manufacturer reports separation performance comparable to commercially available 4–20% Tris-Glycine gradient gels.
The Mini cassette measures 10 × 8.3 cm and contains a gel measuring 8.1 × 7.4 × 0.1 cm. Each gel contains 12 numbered and framed wells with a maximum recommended loading capacity of 25 µL per well. The 12-well configuration provides greater loading capacity per well than the corresponding 15-well Mini gel.
Q-PAGE™ TGN Mini gels are designed primarily for Bio-Rad® Mini-PROTEAN® systems and other electrophoresis tanks compatible with the same Mini cassette dimensions.
The optimized TGN formulation supports rapid electrophoresis. Under suitable conditions, protein separation may be completed in approximately 19 minutes at 300 V. Actual running conditions should be optimized for the electrophoresis apparatus, sample composition and required resolution.
Key Features: Ready-to-use precast polyacrylamide gels • 4–15% gradient for broad-range protein separation • Compact Mini cassette format • 12 numbered and framed wells • 25 µL maximum capacity per well • Conventional Tris-Glycine-SDS buffer compatibility • Rapid electrophoresis • Clear and reproducible protein bands • Migration comparable to traditional Laemmli gels • Compatible with Bio-Rad® and similar Mini systems • Suitable for protein staining and Western blotting • Stable for ambient-temperature transportation
Applications: SDS-PAGE • Broad-Range Protein Separation • Protein Molecular-Weight Analysis • Protein-Purity Assessment • Recombinant Protein Analysis • Protein Expression Studies • Gel Staining • Western Blot Sample Separation • General Proteomics Research
Recommended Running Buffer: Use fresh 1X Tris-Glycine-SDS running buffer. SMOBIO SMOChem™ 1X Tris-Glycine-SDS Running Buffer Powder, Cat. No. BF4000, is compatible with this gel.
Compatible Electrophoresis Systems: Bio-Rad® Mini-PROTEAN® systems • Other electrophoresis systems compatible with 10 × 8.3 cm Mini gel cassettes
Pack Contents: 10 Q-PAGE™ TGN Mini precast gels • Product-description tip card • Gel remover • Cassette opener
Storage: Store the gels flat at 4°C for up to 12 months. Do not freeze. Keep the gels in their original packaging until required for use.
Handling: Remove the cassette sealing tape and comb before electrophoresis. Rinse the wells with running buffer, fill them with running buffer and then load the prepared samples and protein marker.
Research Use: Research Use Only (RUO). Not intended for diagnostic or clinical use.
Manufacturer: SMOBIO Technology, Inc.
Technical Specifications
| Specification | Details |
|---|---|
| Catalogue No. | QP4510 |
| Pack Size | 10 precast gels |
| Product Series | Q-PAGE™ TGN Precast Gel |
| Gel Chemistry | TGN – Tris-Glycine Novel |
| Electrophoresis Method | SDS-PAGE |
| Gel Percentage | 4–15% polyacrylamide gradient |
| Buffer System | Tris-Glycine, Laemmli-compatible |
| Recommended Running Buffer | 1X Tris-Glycine-SDS running buffer |
| Cassette Format | Mini gel cassette |
| Cassette Dimensions | 10 × 8.3 cm |
| Gel Dimensions | 8.1 × 7.4 × 0.1 cm (width × length × thickness) |
| Gel Thickness | 1 mm |
| Number of Wells | 12 |
| Maximum Well Capacity | 25 µL per well |
| Well Design | Numbered and framed wells |
| Compatible Systems | Bio-Rad® Mini-PROTEAN® and compatible Mini electrophoresis systems |
| Rapid-Run Capability | Approximately 19 minutes at 300 V under suitable conditions |
| Downstream Applications | Protein staining, imaging and Western blot transfer |
| Accessories Supplied | Product-description tip card, gel remover and cassette opener |
| Transportation Stability | Stable for shipping at ambient temperature |
| Storage Temperature | 4°C; store flat and do not freeze |
| Stated Shelf Life | Up to 12 months |
| Classification | Research Use Only (RUO) |
Note: Electrophoresis time varies with the equipment, running buffer, gel percentage, sample composition, temperature and applied voltage. Optimize the running conditions for the selected electrophoresis system.
TECHNICAL LIBRARY
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