Schenk & Hildebrandt Basal salt medium

Schenk & Hildebrandt Basal salt medium by Duchefa Biochemie is supplied for plant tissue culture and laboratory research.

Description and application

Schenk en Hildebrandt medium has been developed for growth of both monocotyle and dicotyle cell suspensions. A high level of auxin-type growth regulators, 2,4-D (0.5 mg/l) and 4-CPA (2.0 mg/l), g-enerally favoured monocoty-ledonous cell cul-tu-r-es, while low levels of cytokin, kinetin (0.1 mg/l), were essential for most dicotyledonous cell cultures.

Composition and technical specifications

Micro Elements	mg/l	µM
CoCl2.6H2O	0.10
CuSO4.5H2O	0.20
FeNaEDTA	19.80
H3BO3	5.00
KI	1.00
MnSO4.H2O	10.00
Na2MoO4.2H2O	0.10
ZnSO4.7H2O	1.00

Macro Elements	mg/l	mM
CaCl2	151.00
KNO3	2500.00
MgSO4	195.05
(NH4)H2PO4	300.00

Vitamins	mg/l	µM
myo-Inositol	1000.0
Nicotinic acid	5.0
Pyridoxine HCl	0.5
Thiamine HCl	5.0

Total concentration Micro and Macro elements including vitamins: 4193.75mg/l

Schenk R.U. and Hildebrandt A.C., Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50, 199 (1972).

Hazard statements

• H315 Causes skin irritation
• H319 Causes serious eye irritation
• H335 May cause respiratory irritation

Precautionary statements

• P261 Avoid breathing dust/fume/gas/mist/vapours/spray
• P302 IF ON SKIN:
• P305 IF IN EYES:
• P351 Rinse continuously with water for several minutes
• P352 Wash with soap and water

Available pack options: 10 l, 50 l. Manufacturer reference: S0225.

Safety data sheet