PRODUCT INFORMATION
ExcelTaq™ 2X Fast qPCR Master Mix, SYBR, No ROX, 500 RXN
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PRODUCT INFORMATION
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01 Description, applications & specifications
Description
ExcelTaq™ 2X Fast qPCR Master Mix, SYBR, No ROX is a ready-to-use real-time PCR mixture containing highly stable Hot-Start Taq polymerase, SYBR Green dye and the essential qPCR components except primers and template.
The formulation supports fast and standard cycling programmes, with approximately two minutes of enzyme activation. A smart blue contrast dye assists accurate reaction setup without interfering with fluorescence detection.
Key Features: Ready-to-use 2X formulation • SYBR Green detection chemistry • No ROX reference dye • Fast Hot-Start activation • Fast and standard qPCR programmes • Run times under one hour • High sensitivity and precision • Low fluorescence background • High specificity • Smart blue contrast dye • High storage stability
Applications: Fast SYBR Green qPCR • Standard qPCR • Gene-Expression Analysis • DNA Quantification • Two-Step RT-qPCR • Copy-Number Analysis • Standard-Curve Quantification • Melt-Curve Analysis • High-Throughput qPCR • Molecular Biology Research
Compatibility: Compatible with many real-time PCR instruments that do not require ROX. If passive-reference normalisation is required, add a compatible ROX dye separately or select a ROX-containing formulation.
Packaging: 1 mL × 5 tubes, providing 5 mL total and sufficient reagent for approximately 500 reactions.
Manufacturer: SMOBIO Technology
Technical Specifications
| Specification | Details |
|---|---|
| SKU | TQ1201 |
| Product Name | ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, No ROX) |
| Product Format | Ready-to-use fast qPCR master mix |
| Concentration | 2X |
| Detection Chemistry | SYBR Green double-stranded DNA-binding dye |
| Polymerase | Highly stable, fast-activating Hot-Start Taq DNA polymerase |
| ROX Reference Dye | Not included |
| External ROX Compatibility | Yes; ROX may be added separately when required by the instrument |
| Contrast Dye | Inert smart blue contrast dye |
| Pack Size | 1 mL × 5 tubes; 5 mL total |
| Number of Reactions | Approximately 500 reactions |
| Nominal Reaction Volume | 20 µL |
| Master Mix per 20 µL Reaction | 10 µL |
| Components Added by User | Forward primer, reverse primer, template and nuclease-free water |
| Demonstrated cDNA Range | 25 ng to 1.4 fg under the manufacturer’s test conditions |
| Melt-Curve Analysis | Supported |
| Enzyme Activation Time | Approximately 2 minutes |
| qPCR Programme Type | Fast and standard cycling programmes |
| Fast-Programme Run Time | Less than 1 hour under compatible cycling conditions |
| Reported Time Saving | Up to approximately 60% with a compatible fast programme |
| Stability Evaluation | Performance evaluated after storage at −20°C, 4°C, 22°C and 37°C for 30 days |
| Compatible Instrument Examples | Bio-Rad CFX systems, Qiagen Rotor-Gene systems, Roche LightCycler systems, Thermo Fisher QuantStudio systems and other compatible real-time PCR instruments |
| Storage Temperature | −20°C |
| Stated Shelf Life | 12 months at −20°C |
| Light Protection | Required |
| Freeze–Thaw Precaution | Aliquot to minimise repeated freeze–thaw cycles |
| Manufacturer | SMOBIO Technology |
| Classification | Research Use Only (RUO) |
Note: Confirm whether the selected qPCR instrument requires ROX passive-reference dye. Disable the passive-reference channel when running this master mix without added ROX. Fast-programme performance and total run time depend on the instrument, reaction volume and assay design.
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