PRODUCT INFORMATION
SMOChem™ dNTP Mix, 10 mM Each, 5 × 200 µL
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PRODUCT INFORMATION
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01 Description, applications & specifications
Description
SMOChem™ dNTP Mix, 10 mM Each is a ready-to-use, equimolar mixture of ultrapure dATP, dCTP, dGTP and dTTP. Each nucleotide is supplied at 10 mM, providing a total dNTP concentration of 40 mM.
The premixed formulation reduces pipetting steps and helps minimise reaction-setup errors. This pack contains five 200 µL vials for convenient aliquot use in PCR, real-time PCR, reverse transcription and other DNA-synthesis applications.
Key Features: Four-nucleotide premix • 10 mM of each dNTP • 40 mM total concentration • Equimolar formulation • Ready to use • Five-vial pack • Reduces repeated freeze–thaw exposure • Nuclease and ribonuclease free
Applications: PCR Amplification • Real-Time PCR • Reverse Transcription • One-Step RT-PCR • cDNA Synthesis • DNA Sequencing • DNA Fill-In Reactions • Primer Extension • DNA Labelling
Compatibility: Suitable for molecular biology workflows requiring a balanced mixture of dATP, dCTP, dGTP and dTTP. Final dNTP concentration should be optimised for the selected polymerase, magnesium concentration and assay conditions.
Packaging: Five vials containing 200 µL each; 1,000 µL total volume.
Manufacturer: SMOBIO Technology
Technical Specifications
| Specification | Details |
|---|---|
| SKU | CD1011 |
| Product Name | SMOChem™ Deoxynucleotide (dNTP) Mix |
| Format | Premixed aqueous solution |
| Nucleotide Components | dATP, dCTP, dGTP and dTTP |
| dATP Concentration | 10 mM |
| dCTP Concentration | 10 mM |
| dGTP Concentration | 10 mM |
| dTTP Concentration | 10 mM |
| Total dNTP Concentration | 40 mM |
| Mix Ratio | Equimolar |
| Volume per Vial | 200 µL |
| Number of Vials | 5 |
| Total Supplied Volume | 1,000 µL (1 mL) |
| Formulation pH | pH 8.5 |
| Exodeoxyribonuclease Activity | Not detected |
| Endodeoxyribonuclease Activity | Not detected |
| Ribonuclease Activity | Not detected |
| Primary Applications | PCR, real-time PCR, reverse transcription, DNA sequencing and DNA fill-in reactions |
| Storage Temperature | −20°C |
| Stated Shelf Life | 36 months at −20°C |
| Classification | Research Use Only (RUO) |
Note: dNTP concentration and magnesium concentration are interdependent. Optimise both parameters according to the selected DNA polymerase, template and amplification protocol.
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