SMOChem™ dNTP Mix, 25 mM Each, 500 µL

PRODUCT INFORMATION

SMOChem™ dNTP Mix, 25 mM Each, 500 µL

ManufacturerSMOBIO Catalogue / SKUCD1020
Price £49.95 GBP UK pricing

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PRODUCT INFORMATION

Clear technical information,
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Product typedNTP CollectiondNTP Mixes
01 Description, applications & specifications

Description

SMOChem™ dNTP Mix, 25 mM Each is a concentrated, ready-to-use mixture of ultrapure dATP, dCTP, dGTP and dTTP. Each nucleotide is supplied at 25 mM, providing a total dNTP concentration of 100 mM.

The equimolar premix reduces pipetting steps and helps minimise reaction-setup errors. It is suitable for PCR, real-time PCR, reverse transcription, DNA sequencing and other enzymatic DNA-synthesis applications.

Key Features: Concentrated four-nucleotide premix • 25 mM of each dNTP • 100 mM total concentration • Equimolar formulation • Ready to use • Reduces pipetting steps • Nuclease and ribonuclease free • Suitable for PCR and cDNA synthesis

Applications: PCR Amplification • Real-Time PCR • Reverse Transcription • One-Step RT-PCR • cDNA Synthesis • DNA Sequencing • DNA Fill-In Reactions • Primer Extension • DNA Labelling

Compatibility: Suitable for molecular biology workflows requiring a concentrated, balanced mixture of dATP, dCTP, dGTP and dTTP. Dilute to the required working concentration and optimise according to the selected polymerase and magnesium concentration.

Packaging: One vial containing 500 µL of dNTP Mix.

Manufacturer: SMOBIO Technology

Technical Specifications

Specification Details
SKU CD1020
Product Name SMOChem™ Deoxynucleotide (dNTP) Mix
Format Concentrated premixed aqueous solution
Nucleotide Components dATP, dCTP, dGTP and dTTP
dATP Concentration 25 mM
dCTP Concentration 25 mM
dGTP Concentration 25 mM
dTTP Concentration 25 mM
Total dNTP Concentration 100 mM
Mix Ratio Equimolar
Volume 500 µL
Formulation pH pH 8.5
Exodeoxyribonuclease Activity Not detected
Endodeoxyribonuclease Activity Not detected
Ribonuclease Activity Not detected
Primary Applications PCR, real-time PCR, reverse transcription, DNA sequencing and DNA fill-in reactions
Storage Temperature −20°C
Stated Shelf Life 36 months at −20°C
Classification Research Use Only (RUO)

Note: This is a concentrated dNTP mixture. Calculate the required dilution carefully and optimise the final dNTP and magnesium concentrations for the selected amplification system.

TECHNICAL LIBRARY

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