PRODUCT INFORMATION
SMOChem™ dTTP Solution – Sodium Salt, 100 mM, 25 mL
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PRODUCT INFORMATION
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01 Description, applications & specifications
Description
SMOChem™ dTTP Solution – Sodium Salt is an ultrapure aqueous solution of 2′-deoxythymidine-5′-triphosphate supplied at a nominal concentration of 100 mM in purified water.
The solution has a purity of at least 99% by HPLC and is suitable for PCR, reverse transcription, cDNA synthesis and other enzymatic DNA-synthesis applications requiring an individual source of dTTP.
Key Features: Ultrapure dTTP sodium salt • 100 mM nominal concentration • 100–110 mM specification • ≥99% purity by HPLC • Supplied in purified water • Nuclease and ribonuclease free • Tested for human and bacterial DNA contamination • 36-month shelf life at −20°C
Applications: PCR Amplification • Real-Time PCR • Reverse Transcription • RT-PCR • cDNA Synthesis • DNA Sequencing • DNA Labelling • Primer Extension • Enzymatic DNA Synthesis
Compatibility: Suitable for molecular biology workflows requiring an individual dTTP solution. Final nucleotide and magnesium concentrations should be optimised for the selected polymerase and assay conditions.
Packaging: One bottle containing 25 mL of 100 mM dTTP sodium salt solution.
Manufacturer: SMOBIO Technology
Technical Specifications
| Specification | Details |
|---|---|
| SKU | CD4000 |
| Product Name | SMOChem™ dTTP Solution – Sodium Salt |
| Nucleotide | dTTP |
| Nomenclature | 2′-Deoxythymidine-5′-triphosphate |
| Form | Sodium salt in purified water |
| Appearance | Clear, colourless solution |
| Nominal Concentration | 100 mM |
| Concentration Specification | 100–110 mM |
| Volume | 25 mL |
| Purity | ≥99% by HPLC |
| Molecular Formula, Anion | C₁₀H₁₄N₂O₁₄P₃ |
| pH at 4°C | 8.5 ± 0.1 |
| A250/A260 Ratio | 0.64 ± 0.02 |
| A280/A260 Ratio | 0.74 ± 0.02 |
| A290/A260 Ratio | 0.24 ± 0.02 |
| Magnesium Contamination | ≤5 mM |
| Phosphate Contamination | ≤2 mM |
| Human DNA Contamination | Negative; tested using an 18S rRNA target |
| Bacterial DNA Contamination | Negative; tested using a 23S rRNA target |
| Enzymatic Contaminants | Proteases, DNases, RNases and nicking activity: negative |
| Storage Temperature | −20°C |
| Stated Shelf Life | 36 months at −20°C |
| Classification | Research Use Only (RUO) |
Note: Repeated freeze–thaw cycles should be minimised. Consider dividing the solution into working aliquots where frequent use is expected.
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