SphI

SphI is a Jena Bioscience restriction endonuclease for general laboratory molecular-biology workflows.

Isoschizomers: BbuI, PaeI, SpaHI

• Recognition sequence: 5'– G CATG ↓ C –3' / 3'– C ↑ GTAC G –5'
• Concentration: 10 units/μl
• Source: Streptomyces phaeochromogenes
• Supplied with: 10x Universal Buffer (UB)
• Storage: store at -20 °C avoid freeze/thaw cycles
• Shelf life: 12 months
• Form: liquid (Supplied in 10 mM Tris-HCl pH 7.4, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 400 μg/ml BSA and 50 % [v/v] glycerol)

Documentation: Technical and safety documentation is available from HAYU Biomedical on request.