PRODUCT INFORMATION
Tris Base, Molecular Biology Grade
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PRODUCT INFORMATION
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01 Description, applications & specifications
Description
Tris Base, Molecular Biology Grade is a high-purity buffering reagent used to prepare stable laboratory buffers for molecular biology, electrophoresis and protein applications.
It has an assay specification of 99.80–100.10% and is tested for DNase, RNase and protease contamination, making it suitable for sensitive nucleic acid and protein workflows.
Key Features: Molecular biology grade • 99.80–100.10% assay • DNase free • RNase free • Protease free • Highly soluble in water • Suitable for buffers within approximately pH 7.0–9.0
Applications: TAE and TBE Buffer Preparation • Tris-HCl Buffer Preparation • Electrophoresis • DNA and RNA Purification • Protein Extraction • Western Blotting • Sample Preparation • Molecular Biology Research
Compatibility: Suitable for laboratory formulations requiring Tris free base. The final pH must be adjusted using an appropriate acid according to the selected buffer formulation.
Preparation: Dissolve the required quantity in purified water, adjust to the required pH and bring to the final volume according to the validated protocol. Tris-buffer pH is temperature dependent; measure and adjust the pH at the intended preparation temperature.
Packaging: Available in 100 g, 500 g, 1 kg and 5 kg packs.
Manufacturer: HiMedia Laboratories
Technical Specifications
| Specification | Details |
|---|---|
| SKU | MB029 |
| Product Name | Tris Base, Molecular Biology Grade |
| Chemical Name | Tris(hydroxymethyl)aminomethane |
| Synonym | Tris free base; Trometamol |
| CAS Number | 77-86-1 |
| EC Number | 201-064-4 |
| Molecular Formula | NH₂C(CH₂OH)₃ |
| Molecular Weight | 121.14 g/mol |
| Grade | Molecular biology grade |
| Appearance | Colourless or white to off-white crystals, powder, chunks or solid |
| Solubility in Water | 100 mg soluble in 1 mL |
| pH | 10.00–11.50 for a 5% solution in water at 25°C |
| Assay | 99.80–100.10% on a dry basis |
| Melting Range | 162–176°C |
| Water Content | ≤2.00% by Karl Fischer analysis |
| Insoluble Matter | ≤0.005% |
| Iron | ≤0.0005% |
| Heavy Metals as Lead | ≤0.0005% |
| DNase Activity | None detected |
| RNase Activity | None detected |
| Protease Activity | None detected |
| Storage | Store below 30°C in a tightly closed container |
| Shelf Life | 4 years |
| Manufacturer | HiMedia Laboratories |
Note: Tris-buffer pH changes with temperature. Prepare and adjust the buffer at a controlled temperature appropriate for the intended application.
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